Literature summary for 3.2.1.22 extracted from

Tsukimura, T.; Chiba, Y.; Ohno, K.; Saito, S.; Tajima, Y.; Sakuraba, H.
Molecular mechanism for stabilization of a mutant alpha-galactosidase A involving M51I amino acid substitution by imino sugars (2011), Mol. Genet. Metab., 103, 26-32.

Search for more literature for 3.2.1.22

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli and in Methylotrophic yeast Ogataea minuta TK5-3/Ura cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
M51I	enzymological parameter values are almost the same as those of the wild-type GLA. Km (4-methylumbelliferyl alpha-D-galactoside) similar to wild-type. Mutant enzyme is unstable under neutral pH conditions and acidic ones. The effect of imino sugars including 1-deoxygalactonojirimycin and galactostatin bisulfite on the purified mutant enzyme is examined. The imino sugars improve the stability of the mutant enzyme under both neutral and acidic pH conditions. The results of surface plasmon resonance biosensor assaying suggests that the imino sugars retain their binding activity as to the mutant enzyme under both neutral and acidic pH conditions	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1-deoxygalactonojirimycin	-	Homo sapiens
galactostatin bisulfite	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4	-	4-methylumbelliferyl alpha-D-galactoside	pH 4.6, 37°C, mutant M51I	Homo sapiens
4.5	-	4-methylumbelliferyl alpha-D-galactoside	pH 4.6, 37°C, wild-type	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-D-galactoside + H2O	-	Homo sapiens	4-methylumbelliferone + D-galactose	-	?

Synonyms

Synonyms	Comment	Organism
alpha-galactosidase	-	Homo sapiens
GLA	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.6	-	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.038	-	1-deoxygalactonojirimycin	pH 4.6, 37°C, wild-type	Homo sapiens
0.047	-	1-deoxygalactonojirimycin	pH 4.6, 37°C, mutant M51I	Homo sapiens
0.095	-	galactostatin bisulfite	pH 4.6, 37°C, wild-type	Homo sapiens
0.122	-	galactostatin bisulfite	pH 4.6, 37°C, mutant M51I	Homo sapiens

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Release 2023.1 (January 2023)