Crystallization (Comment) | Organism |
---|---|
the crystal structure of alpha-galactosidase from Lactobacillus acidophilus NCFM (LaMel36A) is determined by single-wavelength anomalous dispersion. In addition, a 1.58-A-resolution crystallographic complex with alpha-D-galactose at substrate binding subsite-1 is determined. LaMel36A has a largeN-terminal twisted beta-sandwich domain, connected by a long alpha-helix to the catalytic (beta/alpha)8-barrel domain, and a C-terminal beta-sheet domain | Lactobacillus acidophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
SDS-PAGE | Lactobacillus acidophilus |
260000 | - |
gel filtration | Lactobacillus acidophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus acidophilus | Q7WWP9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Lactobacillus acidophilus | 4-nitrophenol + alpha-D-galactopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | crystal structure, four identical monomers form a tightly packed tetramer where three monomers contribute to the structural integrity of the active site in each monomer | Lactobacillus acidophilus |
Synonyms | Comment | Organism |
---|---|---|
alpha-galactosidase | - |
Lactobacillus acidophilus |
LaMel36A | - |
Lactobacillus acidophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactobacillus acidophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Lactobacillus acidophilus |