Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | activity enhanced by 2fold | Flavobacterium sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Flavobacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
D480A | mutant shows no activity | Flavobacterium sp. |
D549A | mutant shows no activity | Flavobacterium sp. |
D552A | 81.2% activity compared to wild-type using 4-nitrophenyl-alpha-D-galactopyranoside as a substrate | Flavobacterium sp. |
R556A | 27.6% activity compared to wild-type using 4-nitrophenyl-alpha-D-galactopyranoside as a substrate | Flavobacterium sp. |
S548A | mutant shows no activity | Flavobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | completely inhibits activity | Flavobacterium sp. | |
Cr3+ | partial inhibition | Flavobacterium sp. | |
Fe2+ | partial inhibition | Flavobacterium sp. | |
Hg2+ | completely inhibits activity | Flavobacterium sp. | |
SDS | completely inhibits activity | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.47 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5.5, 45°C | Flavobacterium sp. | |
12.58 | - |
melibiose | pH 5.5, 45°C | Flavobacterium sp. | |
14.76 | - |
stachyose | pH 5.5, 45°C | Flavobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
EDTA | activity enhanced by 2fold | Flavobacterium sp. | |
Mn2+ | activity enhanced by 2fold | Flavobacterium sp. | |
Zn2+ | activity enhanced by 2fold | Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
SDS-PAGE | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Flavobacterium sp. | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
guar gum + H2O | - |
Flavobacterium sp. | ? | - |
? | |
locust bean gum + H2O | - |
Flavobacterium sp. | ? | - |
? | |
melibiose + H2O | - |
Flavobacterium sp. | alpha-D-galactose + alpha-D-glucose | - |
? | |
raffinose + H2O | - |
Flavobacterium sp. | sucrose + alpha-D-galactose | - |
? | |
stachyose + 2 H2O | - |
Flavobacterium sp. | 2 alpha-D-galactose + sucrose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-galactosidase | - |
Flavobacterium sp. |
galA17 | - |
Flavobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Flavobacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 55% activity retained | Flavobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.33 | - |
melibiose | pH 5.5, 45°C | Flavobacterium sp. | |
42.04 | - |
stachyose | pH 5.5, 45°C | Flavobacterium sp. | |
654.6 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5.5, 45°C | Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Flavobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | 55% activity retained | Flavobacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | enzyme is stable over a wide pH range, retaining more than 80% of the initial activity after incubation in buffers ranging from pH 5.0 to 9.0 at 37°C for 1 h | Flavobacterium sp. |