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Literature summary for 3.2.1.22 extracted from

  • Zhou, J.; Shi, P.; Huang, H.; Cao, Y.; Meng, K.; Yang, P.; Zhang, R.; Chen, X.; Yao, B.
    A new beta-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for beta-galactosidase activity of gastrointestinal bacteria (2010), Appl. Microbiol. Biotechnol., 88, 1297-1309.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol activity enhanced by 2fold Flavobacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Flavobacterium sp.

Protein Variants

Protein Variants Comment Organism
D480A mutant shows no activity Flavobacterium sp.
D549A mutant shows no activity Flavobacterium sp.
D552A 81.2% activity compared to wild-type using 4-nitrophenyl-alpha-D-galactopyranoside as a substrate Flavobacterium sp.
R556A 27.6% activity compared to wild-type using 4-nitrophenyl-alpha-D-galactopyranoside as a substrate Flavobacterium sp.
S548A mutant shows no activity Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ completely inhibits activity Flavobacterium sp.
Cr3+ partial inhibition Flavobacterium sp.
Fe2+ partial inhibition Flavobacterium sp.
Hg2+ completely inhibits activity Flavobacterium sp.
SDS completely inhibits activity Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.47
-
4-nitrophenyl-alpha-D-galactopyranoside pH 5.5, 45°C Flavobacterium sp.
12.58
-
melibiose pH 5.5, 45°C Flavobacterium sp.
14.76
-
stachyose pH 5.5, 45°C Flavobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
EDTA activity enhanced by 2fold Flavobacterium sp.
Mn2+ activity enhanced by 2fold Flavobacterium sp.
Zn2+ activity enhanced by 2fold Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
SDS-PAGE Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
Flavobacterium sp. 4-nitrophenol + alpha-D-galactopyranose
-
?
guar gum + H2O
-
Flavobacterium sp. ?
-
?
locust bean gum + H2O
-
Flavobacterium sp. ?
-
?
melibiose + H2O
-
Flavobacterium sp. alpha-D-galactose + alpha-D-glucose
-
?
raffinose + H2O
-
Flavobacterium sp. sucrose + alpha-D-galactose
-
?
stachyose + 2 H2O
-
Flavobacterium sp. 2 alpha-D-galactose + sucrose
-
?

Synonyms

Synonyms Comment Organism
alpha-galactosidase
-
Flavobacterium sp.
galA17
-
Flavobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
assay at Flavobacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 55% activity retained Flavobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.33
-
melibiose pH 5.5, 45°C Flavobacterium sp.
42.04
-
stachyose pH 5.5, 45°C Flavobacterium sp.
654.6
-
4-nitrophenyl-alpha-D-galactopyranoside pH 5.5, 45°C Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 7 55% activity retained Flavobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 enzyme is stable over a wide pH range, retaining more than 80% of the initial activity after incubation in buffers ranging from pH 5.0 to 9.0 at 37°C for 1 h Flavobacterium sp.