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Literature summary for 3.2.1.22 extracted from

  • Ferreira, J.G.; Reis, A.P.; Guimaraes, V.M.; Falkoski, D.L.; Fialho, L.d.a..S.; de Rezende, S.T.
    Purification and characterization of Aspergillus terreus alpha-galactosidases and their use for hydrolysis of soymilk oligosaccharides (2011), Appl. Biochem. Biotechnol., 164, 1111-1125.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ inhibits enzymatic activity Aspergillus terreus
Cu2+ inhibits enzymatic activity Aspergillus terreus
D-galactose inhibits enzymatic activity Aspergillus terreus
Hg2+ inhibits enzymatic activity Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
4-nitrophenyl-alpha-D-galactopyranoside pH 5, 40°C Aspergillus terreus
0.66
-
4-nitrophenyl-alpha-D-galactopyranoside pH 5, 40°C Aspergillus terreus
0.72
-
4-nitrophenyl-alpha-D-galactopyranoside pH 5, 40°C Aspergillus terreus
1.92
-
melibiose pH 5, 40°C Aspergillus terreus
7.39
-
melibiose pH 5, 40°C Aspergillus terreus
10.94
-
stachyose pH 5, 40°C Aspergillus terreus
20.14
-
melibiose pH 5, 40°C Aspergillus terreus
27.93
-
raffinose pH 5, 40°C Aspergillus terreus
32.99
-
raffinose pH 5, 40°C Aspergillus terreus
54.74
-
stachyose pH 5, 40°C Aspergillus terreus

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ inhibits enzymatic activity Aspergillus terreus
Cu2+ inhibits enzymatic activity Aspergillus terreus
Hg2+ inhibits enzymatic activity Aspergillus terreus
SDS inhibits enzymatic activity Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Aspergillus terreus
51700
-
native molecular mass Aspergillus terreus
72300
-
SDS-PAGE Aspergillus terreus
77300
-
gel filtration Aspergillus terreus
350000
-
gel filtration Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
Aspergillus terreus 4-nitrophenol + alpha-D-galactopyranose
-
?
guar gum + H2O
-
Aspergillus terreus ?
-
?
locust bean gum + H2O
-
Aspergillus terreus ?
-
?
melibiose + H2O
-
Aspergillus terreus alpha-D-galactose + alpha-D-glucose
-
?
raffinose + H2O
-
Aspergillus terreus sucrose + alpha-D-galactose
-
?
stachyose + 2 H2O
-
Aspergillus terreus 2 alpha-D-galactose + sucrose
-
?
sucrose + H2O
-
Aspergillus terreus alpha-D-glucose + D-fructose
-
?

Subunits

Subunits Comment Organism
homopentamer 5 * 72300, SDS-PAGE Aspergillus terreus
monomer 1 * 50000, SDS-PAGE Aspergillus terreus

Synonyms

Synonyms Comment Organism
E1 alpha-galactosidase
-
Aspergillus terreus
E2 alpha-galactosidase
-
Aspergillus terreus
E3 alpha-galactosidase
-
Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Aspergillus terreus
50
-
optimal temperature Aspergillus terreus
55
-
optimal temperature Aspergillus terreus
60
-
optimal temperature Aspergillus terreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
retains 90% of its initial activity after 70 h at 50°C Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus terreus
5
-
assay at Aspergillus terreus
5.5
-
highest activity Aspergillus terreus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7 stable for 6 h Aspergillus terreus