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Literature summary for 3.2.1.22 extracted from
- Characterization of Bacillus halodurans alpha-galactosidase Mel4A encoded by the mel4A gene (BH2228) (2008), Biosci. Biotechnol. Biochem., 72, 2459-2462.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | or similar reducing agent, required | Halalkalibacterium halodurans |  |
| NAD+ | strong activation | Halalkalibacterium halodurans | |
| NADP+ | slight activation | Halalkalibacterium halodurans | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Halalkalibacterium halodurans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | strong activation | Halalkalibacterium halodurans | |
| Fe2+ | strong activation | Halalkalibacterium halodurans | |
| Mn2+ | strong activation | Halalkalibacterium halodurans | |
| Ni2+ | moderate activation | Halalkalibacterium halodurans | |
| Zn2+ | moderate activation | Halalkalibacterium halodurans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 51000, SDS-PAGE | Halalkalibacterium halodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halalkalibacterium halodurans | Q9KAQ9 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.4 | - |
pH 7.4, 37°C, presence of NAD+, Mn2+, dithiothreitol | Halalkalibacterium halodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Halalkalibacterium halodurans | 4-nitrophenol + D-galactopyranose | - |
? | |
| additional information | no substrate: galactomannan | Halalkalibacterium halodurans | ? | - |
? | |
| raffinose + H2O | - |
Halalkalibacterium halodurans | sucrose + D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000, SDS-PAGE | Halalkalibacterium halodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mel4A | - |
Halalkalibacterium halodurans |
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