Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strong inhibition at 10 mM | Thermomyces lanuginosus | |
Co2+ | slight inhibition at 10 mM | Thermomyces lanuginosus | |
Cu2+ | slight inhibition at 10 mM | Thermomyces lanuginosus | |
Hg+ | complete inactivation at 10 mM | Thermomyces lanuginosus | |
Ni2+ | slight inhibition at 10 mM | Thermomyces lanuginosus | |
Zn2+ | strong inhibition at 10 mM | Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.13 | - |
p-nitrophenyl alpha-D-galactopyranoside | at 55°C, in 50 mM citrate buffer (pH 4.2) | Thermomyces lanuginosus | |
1.17 | - |
stachyose | at 55°C, in 50 mM citrate buffer (pH 4.2) | Thermomyces lanuginosus | |
1.61 | - |
raffinose | at 55°C, in 50 mM citrate buffer (pH 4.2) | Thermomyces lanuginosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates at 10 mM | Thermomyces lanuginosus | |
Mg2+ | activates at 10 mM | Thermomyces lanuginosus | |
Mn2+ | activates at 10 mM | Thermomyces lanuginosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
native enzyme, SDS-PAGE | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
- |
- |
Thermomyces lanuginosus CBS 395.62/b | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the total carbohydrate content of the purified enzyme is 5.3% | Thermomyces lanuginosus |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, Sepharose CL-6B column chromatography, DEAE-Sepharose FF column chromatography, and Superose 12 gel filtration | Thermomyces lanuginosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.9 | - |
unpurified enzyme from fermentation broth, using p-nitrophenyl alpha-D-galactopyranoside as a substrate | Thermomyces lanuginosus |
1813 | - |
after 114fold purification, using p-nitrophenyl alpha-D-galactopyranoside as a substrate | Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermomyces lanuginosus | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermomyces lanuginosus CBS 395.62/b | p-nitrophenol + alpha-D-galactose | - |
? | |
raffinose + H2O | - |
Thermomyces lanuginosus | sucrose + D-galactose | - |
? | |
raffinose + H2O | - |
Thermomyces lanuginosus CBS 395.62/b | sucrose + D-galactose | - |
? | |
stachyose + 2 H2O | - |
Thermomyces lanuginosus | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | - |
Thermomyces lanuginosus CBS 395.62/b | 2 alpha-D-galactose + sucrose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
retains more than 90% of the initial activity at 45°C in a pH range from 5.5-9.0 | Thermomyces lanuginosus |
65 | - |
5 min incubation at 65°C results in complete inactivation of the purified enzyme | Thermomyces lanuginosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3914 | - |
p-nitrophenyl alpha-D-galactopyranoside | at 55°C, in 50 mM citrate buffer (pH 4.2) | Thermomyces lanuginosus | |
6946 | - |
raffinose | at 55°C, in 50 mM citrate buffer (pH 4.2) | Thermomyces lanuginosus | |
7659 | - |
stachyose | at 55°C, in 50 mM citrate buffer (pH 4.2) | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Thermomyces lanuginosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | retains more than 90% of the initial activity at 45°C in a pH range from 5.5-9.0 | Thermomyces lanuginosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomyces lanuginosus | isoelectric focusing | - |
3.9 |