Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21(DE3) cells | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
D220A | exhibits less than 1% of the wild-type enzyme activity | Thermotoga maritima |
D327A | exhibits less than 1% of the wild-type enzyme activity | Thermotoga maritima |
D327G | mutant has a 200-800fold lower catalytic rate on aryl galactosides relative to the wild type enzyme | Thermotoga maritima |
D387A | exhibits less than 1% of the wild-type enzyme activity | Thermotoga maritima |
D387G | mutant is 1500fold catalytically slower than the wild type enzyme on p-nitrophenyl alpha-D-galactopyranoside, and exhibits higher activity with increasing pH | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
2,4-dinitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.039 | - |
beta-naphthyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.048 | - |
4-O-methylphenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.08 | - |
4-nitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.083 | - |
4-nitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.086 | - |
3,4-dimethylphenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.088 | - |
4-O-methylphenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.089 | - |
3,4-dimethylphenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.096 | - |
3-nitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.104 | - |
4-bromophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.111 | - |
4-bromophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.133 | - |
2-nitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.135 | - |
2,4-dinitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.14 | - |
3-nitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.143 | - |
2-nitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.143 | - |
2,5-dinitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.176 | - |
phenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.225 | - |
beta-naphthyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.243 | - |
2,5-dinitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.324 | - |
phenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | O33835 | - |
- |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | O33835 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | 2,4-dinitrophenol + alpha-D-galactose | - |
? | |
2,4-dinitrophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 2,4-dinitrophenol + alpha-D-galactose | - |
? | |
2,5-dinitrophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | 2,5-dinitrophenol + alpha-D-galactose | - |
? | |
2,5-dinitrophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 2,5-dinitrophenol + alpha-D-galactose | - |
? | |
2-nitrophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | 2-nitrophenol + alpha-D-galactose | - |
? | |
2-nitrophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 2-nitrophenol + alpha-D-galactose | - |
? | |
3,4-dimethylphenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | 3,4-dimethylphenol + alpha-D-galactose | - |
? | |
3,4-dimethylphenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 3,4-dimethylphenol + alpha-D-galactose | - |
? | |
3-nitrophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | 3-nitrophenol + alpha-D-galactose | - |
? | |
4-bromophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | 4-bromophenol + alpha-D-galactose | - |
? | |
4-methoxyphenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | 4-methoxyphenol + alpha-D-galactose | - |
? | |
4-nitrophenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + alpha-D-galactose | - |
? | |
beta-naphthyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | beta-naphthol + alpha-D-galactose | - |
? | |
additional information | no detectable enzymatic activity on xylo-, fuco-, manno-, or glucopyranosides | Thermotoga maritima | ? | - |
? | |
additional information | no detectable enzymatic activity on xylo-, fuco-, manno-, or glucopyranosides | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
phenyl alpha-D-galactoside + H2O | - |
Thermotoga maritima | phenol + alpha-D-galactose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GALA | - |
Thermotoga maritima |
GH36 alpha-galactosidase | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
89.8 | - |
melting temperature of the wild type enzyme | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
phenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.01 | - |
4-O-methylphenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.01 | - |
3,4-dimethylphenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.016 | - |
4-bromophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.021 | - |
beta-naphthyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.027 | - |
phenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.03 | 0.55 | 4-O-methylphenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.03 | - |
4-nitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.035 | - |
2-nitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.042 | - |
2,5-dinitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.042 | - |
3-nitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.052 | 2.1 | 3,4-dimethylphenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.085 | - |
2,4-dinitrophenyl alpha-D-galactoside | mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.41 | - |
2,4-dinitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
0.52 | - |
2-nitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
5.71 | - |
phenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
6.26 | - |
beta-naphthyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
6.69 | - |
4-O-methylphenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
8.58 | - |
4-nitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
10.39 | - |
3,4-dimethylphenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
10.5 | - |
3-nitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
11.2 | - |
2,5-dinitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
12.23 | - |
2,4-dinitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
12.58 | - |
4-bromophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima | |
13.67 | - |
2-nitrophenyl alpha-D-galactoside | wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C | Thermotoga maritima |