Literature summary for 3.2.1.22 extracted from

Yasuda, K.; Chang, H.H.; Wu, H.L.; Ishii, S.; Fan, J.Q.
Efficient and rapid purification of recombinant human alpha-galactosidase A by affinity column chromatography (2004), Protein Expr. Purif., 37, 499-506.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Sf9 cells infected with recombinant baculovirus, expression in COS-7 cells	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
efficient and rapid purification procedure for recombinant alpha-galactosidase A by sequential Concanavalin A-Sepharose and immobilized thio-alpha-galactoside agarose column chromatography. This procedure is especially useful for the purification of mutant forms of alpha-galactosidase A, which are not stable under conventional purification techniques. Purification of the intracellular mutant enzyme M279I, expressed in COS-7 cells within 6 h at 62% overall yield	Homo sapiens

Purification (Comment)

Organism

efficient and rapid purification procedure for recombinant alpha-galactosidase A by sequential Concanavalin A-Sepharose and immobilized thio-alpha-galactoside agarose column chromatography. This procedure is especially useful for the purification of mutant forms of alpha-galactosidase A, which are not stable under conventional purification techniques. Purification of the intracellular mutant enzyme M279I, expressed in COS-7 cells within 6 h at 62% overall yield

Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-D-galactoside + H2O	-	Homo sapiens	alpha-D-galactose + 4-methylumbelliferone	-	?

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Release 2023.1 (January 2023)