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Literature summary for 3.2.1.22 extracted from
- Purification, cloning, and properties of alpha-galactosidase from Saccharopolyspora erythraea and its use as a reporter system (2005), Appl. Microbiol. Biotechnol., 67, 91-96.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | alpha-galactosidase is used as a simple yet powerful reporter enzyme system in Saccharopolyspora erythraea. This reporter sytem has the distinct advantage of quantitatively measuring the level of alpha-galactosidase activity quickly and easily from culture supernatant | Saccharopolyspora erythraea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Saccharopolyspora erythraea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
p-nitrophenyl-alpha-D-galactoside | pH 6, 36°C | Saccharopolyspora erythraea |  |
| 0.65 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 6.0, 36°C | Saccharopolyspora erythraea | |
| 1.4 | - |
melibiose | pH 6, 36°C | Saccharopolyspora erythraea | |
| 1.4 | - |
melibiose | pH 6.0, 36°C | Saccharopolyspora erythraea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Saccharopolyspora erythraea | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | 34000 | gel filtration | Saccharopolyspora erythraea |
| 45000 | - |
1 * 45000, SDS-PAGE | Saccharopolyspora erythraea |
| 45000 | - |
x * 45000, SDS-PAGE | Saccharopolyspora erythraea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharopolyspora erythraea | Q9Z4N5 | - |
- |
| Saccharopolyspora erythraea | Q9Z4N5 | alpha-galactosidase precursor | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharopolyspora erythraea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Saccharopolyspora erythraea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Saccharopolyspora erythraea | 4-nitrophenol + D-galactopyranose | - |
? | |
| melibiose + H2O | - |
Saccharopolyspora erythraea | D-galactose + D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-galactoside + H2O | - |
Saccharopolyspora erythraea | p-nitrophenol + alpha-D-galactose | - |
? | |
| raffinose + H2O | - |
Saccharopolyspora erythraea | sucrose + D-galactose | - |
? | |
| stachyose + H2O | - |
Saccharopolyspora erythraea | ? | - |
? | |
| stachyose + H2O | - |
Saccharopolyspora erythraea | raffinose + D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Saccharopolyspora erythraea |
| monomer | 1 * 45000, SDS-PAGE | Saccharopolyspora erythraea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
10 min, 15% loss of activity | Saccharopolyspora erythraea |
| 65 | - |
10 min, 85% residual activity | Saccharopolyspora erythraea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
- |
Saccharopolyspora erythraea |
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