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Literature summary for 3.2.1.22 extracted from

  • Kim, W.D.; Kobayashi, O.; Kaneko, S.; Sakakibara, Y.; Park, G.G.; Kusakabe, I.; Tanaka, H.; Kobayashi, H.
    alpha-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells (2002), Phytochemistry, 61, 621-630.
    View publication on PubMed
Search for more literature for 3.2.1.22

Cloned(Commentary)

Cloned (Comment) Organism
-
 Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ 1 mM, 46% inhibition Oryza sativa
Hg2+ 1 mM, 98% inhibition Oryza sativa
PCMB 1 mM, 98% inhibition Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39950
-
 1 * 39950, calculation from nucleotide sequence Oryza sativa
40000
-
 gel filtration Oryza sativa
41000
-
 1 * 41000, SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q9FXT4 L. var. Nipponbare
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
119.7
-
-
 Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galactopyranosyl-(1-6)-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
-
 Oryza sativa alpha-D-galactose + beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose
-
 ?
melibiose + H2O
-
 Oryza sativa alpha-D-galactose + alpha-D-glucose
-
 ?
O-beta-D-mannopyranosyl-(1-4)-O-[alpha-D-galactopyranosyl-(1-6)]-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
-
 Oryza sativa ?
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Oryza sativa p-nitrophenol + alpha-D-galactose
-
 ?
raffinose + H2O
-
 Oryza sativa sucrose + alpha-D-galactose
-
 ?
stachyose + 2 H2O
-
 Oryza sativa 2 alpha-D-galactose + sucrose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Oryza sativa
monomer 1 * 39950, calculation from nucleotide sequence Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 activity with p-nitrophenyl alpha-D-galactopyranoside Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 inactivation above Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Oryza sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 inactivation below pH 3 and above pH 8 Oryza sativa