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Literature summary for 3.2.1.22 extracted from

  • Puchart, V.; Vrsanska, M.; Bhat, M.K.; Biely, P.
    Purification and characterization of alpha-galactosidase from a thermophilic fungus Thermomyces lanuginosus (2000), Biochim. Biophys. Acta, 1524, 27-37.
    View publication on PubMed
Search for more literature for 3.2.1.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 4-methylumbelliferyl alpha-D-galactoside 50°C, pH 4.5 Thermomyces lanuginosus
2.4
-
 melibiose 50°C, pH 4.5 Thermomyces lanuginosus
11.3
-
 raffinose 50°C, pH 4.5 Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermomyces lanuginosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, SDS-PAGE Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
 IMI 158749
-
Thermomyces lanuginosus IMI 158749
-
 IMI 158749
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Thermomyces lanuginosus

Purification (Commentary)

Purification (Comment) Organism
-
 Thermomyces lanuginosus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Thermomyces lanuginosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
266.6
-
-
 Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-galactoside + H2O
-
 Thermomyces lanuginosus 4-methylumbelliferone + alpha-D-galactose
-
 ?
4-methylumbelliferyl alpha-D-galactoside + H2O
-
 Thermomyces lanuginosus IMI 158749 4-methylumbelliferone + alpha-D-galactose
-
 ?
melibiose + H2O
-
 Thermomyces lanuginosus alpha-D-galactose + D-glucose
-
 ?
melibiose + H2O
-
 Thermomyces lanuginosus IMI 158749 alpha-D-galactose + D-glucose
-
 ?
raffinose + H2O
-
 Thermomyces lanuginosus sucrose + alpha-D-galactose
-
 ?
raffinose + H2O
-
 Thermomyces lanuginosus IMI 158749 sucrose + alpha-D-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65 70
-
 Thermomyces lanuginosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 75 50°C: about 60% of maximal activity, 75°C: about 50% of maximal activity Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 4.5, 50 mM sodium acetate buffer, stable for 6 h Thermomyces lanuginosus
65
-
 pH 4.5, 50 mM sodium acetate buffer, about 30% loss of activity after 2 h, about 50% loss of activitry after 3 h, about 90% loss of activity after 6 h Thermomyces lanuginosus
70
-
 pH 4.5, 50 mM sodium acetate buffer, about 75% loss of activity after 30 min Thermomyces lanuginosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
 melibiose 50°C, pH 4.5 Thermomyces lanuginosus
32.4
-
 raffinose 50°C, pH 4.5 Thermomyces lanuginosus
49.8
-
 4-methylumbelliferyl alpha-D-galactoside 50°C, pH 4.5 Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Thermomyces lanuginosus

pH Range

pH Minimum pH Maximum Comment Organism
2 6 pH 2.0: about 50% of maximal activity, pH 6.0: about 50% of maximal activity Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7.5 50°C, 5 h, stable Thermomyces lanuginosus

pI Value

Organism Comment pI Value Maximum pI Value
Thermomyces lanuginosus isoelectric focusing, pH-gradient 3-7
-
 4.9