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Literature summary for 3.2.1.22 extracted from
- Purification and characterization of two alpha-galactosidases associated with catabolism of guar gum and other alpha-galactosides by Bacteroides ovatus (1985), J. Bacteriol., 161, 500-506.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
o-nitrophenyl-beta-D-galactopyranose | alpha-galactosidase II | Bacteroides ovatus |  |
| 0.2 | - |
p-nitrophenyl-beta-D-galactopyranose | alpha-galactosidase II | Bacteroides ovatus | |
| 0.3 | - |
stachyose | alpha-galactosidase II | Bacteroides ovatus | |
| 0.3 | - |
o-nitrophenyl-beta-D-galactopyranose | alpha-galactosidase II | Bacteroides ovatus | |
| 0.4 | - |
p-nitrophenyl-beta-D-galactopyranose | alpha-galactosidase II | Bacteroides ovatus | |
| 2.3 | - |
melibiose | alpha-galactosidase II | Bacteroides ovatus | |
| 5.9 | - |
raffinose | alpha-galactosidase II | Bacteroides ovatus | |
| 8.5 | - |
stachyose | alpha-galactosidase I | Bacteroides ovatus | |
| 20.8 | - |
melibiose | alpha-galactosidase I | Bacteroides ovatus | |
| 98.1 | - |
raffinose | alpha-galactosidase I | Bacteroides ovatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80500 | - |
alpha-galactosidase II, 3 * 80500 | Bacteroides ovatus |
| 85000 | - |
alpha-galactosidase I, 3 * 85000 | Bacteroides ovatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides ovatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteroides ovatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02 | - |
D-glucose | Bacteroides ovatus |
| 0.08 | - |
D-mannose | Bacteroides ovatus |
| 0.13 | - |
D-galactose | Bacteroides ovatus |
| 0.26 | - |
melibiose | Bacteroides ovatus |
| 0.3 | - |
raffinose | Bacteroides ovatus |
| 0.41 | - |
guar gum | Bacteroides ovatus |
| 0.42 | - |
stachyose | Bacteroides ovatus |
| 41 | - |
alpha-galactosidase II | Bacteroides ovatus |
| 129 | - |
alpha-galactosidase I | Bacteroides ovatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| melibiose + H2O | - |
Bacteroides ovatus | D-galactose + D-glucose | - |
? | |
| o-nitrophenyl-beta-D-galactopyranose + H2O | - |
Bacteroides ovatus | o-nitrophenol + beta-D-galactopyranose | - |
? | |
| p-nitrophenyl-beta-D-galactopyranose + H2O | - |
Bacteroides ovatus | ? | - |
? | |
| raffinose + H2O | - |
Bacteroides ovatus | sucrose + D-galactose | - |
? | |
| stachyose + H2O | - |
Bacteroides ovatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | alpha-galactosidase I, 3 * 85000 | Bacteroides ovatus |
| trimer | alpha-galactosidase II, 3 * 80500 | Bacteroides ovatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | 6.4 | alpha-galactosidase I | Bacteroides ovatus |
| 6.3 | 6.5 | alpha-galactosidase II | Bacteroides ovatus |
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