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Literature summary for 3.2.1.22 extracted from
- alpha-Galactosidases of Vigna unguiculata (1989), Phytochemistry, 28, 2047-2051.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
p-nitrophenyl alpha-D-galactoside | alpha-galactosidase I | Vigna unguiculata |  |
| 1.6 | - |
raffinose | alpha-galactosidase II2 | Vigna unguiculata | |
| 2.2 | - |
p-nitrophenyl alpha-D-galactoside | alpha-galactosidase II2 | Vigna unguiculata | |
| 4.6 | - |
raffinose | alpha-galactosidase I | Vigna unguiculata | |
| 5 | - |
raffinose | alpha-galactosidase II1 | Vigna unguiculata | |
| 5.3 | - |
p-nitrophenyl alpha-D-galactoside | alpha-galactosidase II1 | Vigna unguiculata | |
| 11 | - |
stachyose | alpha-galactosidase I | Vigna unguiculata | |
| 15 | - |
stachyose | alpha-galactosidase II1 | Vigna unguiculata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
alpha-galactosidase II1, gel filtration | Vigna unguiculata |
| 30000 | - |
alpha-galactosidase II2, gel filtration | Vigna unguiculata |
| 31500 | - |
alpha-galactosidase I, 4 * 31500, SDS-PAGE | Vigna unguiculata |
| 111000 | - |
alpha-galactosidase I, gel filtration | Vigna unguiculata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna unguiculata | - |
cowpea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna unguiculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-D-galactoside + H2O | - |
Vigna unguiculata | p-nitrophenol + alpha-D-galactose | - |
? | |
| raffinose + H2O | - |
Vigna unguiculata | sucrose + D-galactose | - |
? | |
| stachyose + H2O | - |
Vigna unguiculata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha-galactosidase I, 4 * 31500, SDS-PAGE | Vigna unguiculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
alpha-galactosidase I | Vigna unguiculata |
| 5.3 | - |
alpha-galactosidase II2 | Vigna unguiculata |
| 5.9 | - |
alpha-galactosidase II1 | Vigna unguiculata |
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Release 2023.1 (January 2023)
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