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Literature summary for 3.2.1.212 extracted from

  • Thanassi, N.M.; Nakada, H.I.
    Enzymic degradation of fucoidan by enzymes from the hepatopancreas of abalone, Halotus species (1967), Arch. Biochem. Biophys., 118, 172-177 .
No PubMed abstract available
Search for more literature for 3.2.1.212

Activating Compound

Activating Compound Comment Organism Structure
cysteine 0.01 M, 1.05fold activation Haliotis sp.
N-ethylmaleimide 0.01 M, 1.4fold activation Haliotis sp.

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 0.001 M, 58% loss of activity Haliotis sp.
cysteine 0.001 M, 7% loss of activity Haliotis sp.
EDTA 0.02 M, 22% loss of activity Haliotis sp.
HgCl2 0.001 M, 80% loss of activity Haliotis sp.
MnCl2 0.001, 24% loss of activity Haliotis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
MgSO4 0.01 M, 1.2fold activation Haliotis sp.

Organism

Organism UniProt Comment Textmining
Haliotis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Haliotis sp.

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Haliotis sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fucoidan + H2O fucoidan from Fucus gardneri. The fucoidanase hydrolyzes fucoidan without desulfation to form oligosaccharides ranging from 10 to 2 fucose units plus the monosaccharide, fucose. Fucose can not be detected until late in the hydrolysis period, and sulfate release is not detectable during the first 4 hours of fucoidanase action Haliotis sp. sulfated alpha-L-fucooligosaccharides
-
 ?
additional information no degradation of 4-nitrophenyl-L-fucoside or blood group substance Haliotis sp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Haliotis sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 30% of maximal activity, 50°C: about 80% of maximal activity Haliotis sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 5 min, 15% loss of activity Haliotis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 citrate buffer, phosphate buffer, or citrate phosphate Haliotis sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 pH 4.5: about 75% of maximal activity, pH 6.5: about 40% of maximal activity Haliotis sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 30 min at room temperature, 60% loss of activity Haliotis sp.
5 8.5 30 min at room temperature, stable Haliotis sp.
9
-
 30 min at room temperature, 60% loss of activity Haliotis sp.