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Literature summary for 3.2.1.21 extracted from

  • Liew, K.J.; Lim, L.; Woo, H.Y.; Chan, K.G.; Shamsir, M.S.; Goh, K.M.
    Purification and characterization of a novel GH1 beta-glucosidase from Jeotgalibacillus malaysiensis (2018), Int. J. Biol. Macromol., 115, 1094-1102 .
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
D-glucose the enzyme is activated by up to 250 mM D-glucose Jeotgalibacillus malaysiensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Jeotgalibacillus malaysiensis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 61% residual activity at 5 mM Jeotgalibacillus malaysiensis
Co2+ 4% residual activity at 5 mM Jeotgalibacillus malaysiensis
Cu2+ complete inhibition at 5 mM Jeotgalibacillus malaysiensis
D-glucose the enzyme is able to tolerate D-glucose concentration up to 2500 mM, retaining about 80% of its maximal activity Jeotgalibacillus malaysiensis
DMSO 84% residual activity at 5% (v/v) Jeotgalibacillus malaysiensis
EDTA 4% residual activity at 5 mM Jeotgalibacillus malaysiensis
Fe3+ 6% residual activity at 5 mM Jeotgalibacillus malaysiensis
Mn2+ 7% residual activity at 5 mM Jeotgalibacillus malaysiensis
Ni2+ 3% residual activity at 5 mM Jeotgalibacillus malaysiensis
Rb+ 77% residual activity at 5 mM Jeotgalibacillus malaysiensis
SDS 2% residual activity at 5 mM Jeotgalibacillus malaysiensis
Triton X-100 70% residual activity at 5% (v/v) Jeotgalibacillus malaysiensis
Tween 20 75% residual activity at 5% (v/v) Jeotgalibacillus malaysiensis
Tween 80 47% residual activity at 5% (v/v) Jeotgalibacillus malaysiensis
Urea 85% residual activity at 5 mM Jeotgalibacillus malaysiensis
Zn2+ 7% residual activity at 5 mM Jeotgalibacillus malaysiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 4-nitrophenyl beta-D-glucopyranoside at pH 7.4 and 65°C Jeotgalibacillus malaysiensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 121% activity at 5 mM. The activity and stability are increased when CaCl2 is supplemented to the enzyme Jeotgalibacillus malaysiensis
additional information not influenced by Na+, K+ and Mg2+ Jeotgalibacillus malaysiensis

Organism

Organism UniProt Comment Textmining
Jeotgalibacillus malaysiensis
-
-
-
Jeotgalibacillus malaysiensis DSM 28777T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Jeotgalibacillus malaysiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Jeotgalibacillus malaysiensis 4-nitrophenol + beta-D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Jeotgalibacillus malaysiensis DSM 28777T 4-nitrophenol + beta-D-glucopyranose
-
 ?
cellobiose + H2O 100% activity Jeotgalibacillus malaysiensis 2 beta-D-glucose
-
 ?
cellobiose + H2O 100% activity Jeotgalibacillus malaysiensis DSM 28777T 2 beta-D-glucose
-
 ?
cellohexanose + H2O 85% activity compared to cellobiose Jeotgalibacillus malaysiensis beta-D-glucose + ?
-
 ?
cellopentose + H2O 125% activity compared to cellobiose Jeotgalibacillus malaysiensis beta-D-glucose + ?
-
 ?
cellotetraose + H2O 125% activity compared to cellobiose Jeotgalibacillus malaysiensis beta-D-glucose + ?
-
 ?
cellotetraose + H2O 125% activity compared to cellobiose Jeotgalibacillus malaysiensis DSM 28777T beta-D-glucose + ?
-
 ?
cellotriose + H2O 124% activity compared to cellobiose Jeotgalibacillus malaysiensis beta-D-glucose + ?
-
 ?
gentibiose + H2O 54% activity compared to cellobiose Jeotgalibacillus malaysiensis 2 beta-D-glucose
-
 ?
lactose + H2O 34% activity compared to cellobiose Jeotgalibacillus malaysiensis beta-D-glucose + D-galactose
-
 ?
additional information not active towards sucrose and maltose as well as polysaccharides such as Avicel, carboxymethyl cellulose, Sigmacell cellulose 101, alpha-cellulose and xylan Jeotgalibacillus malaysiensis ?
-
 ?
additional information not active towards sucrose and maltose as well as polysaccharides such as Avicel, carboxymethyl cellulose, Sigmacell cellulose 101, alpha-cellulose and xylan Jeotgalibacillus malaysiensis DSM 28777T ?
-
 ?
salicin + H2O 118% activity compared to cellobiose Jeotgalibacillus malaysiensis beta-D-glucose + ?
-
 ?
salicin + H2O 118% activity compared to cellobiose Jeotgalibacillus malaysiensis DSM 28777T beta-D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Jeotgalibacillus malaysiensis

Synonyms

Synonyms Comment Organism
beta-D-glucosidase
-
 Jeotgalibacillus malaysiensis
Bgl
-
 Jeotgalibacillus malaysiensis
BglD5
-
 Jeotgalibacillus malaysiensis
cellobiase
-
 Jeotgalibacillus malaysiensis
gentiobiase
-
 Jeotgalibacillus malaysiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Jeotgalibacillus malaysiensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 75 more than 50% activity between 50 and 75°C Jeotgalibacillus malaysiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the half-life at 65°C without Ca2+ is 35 min, the half-life at 65°C wit Ca2+ is 70 min Jeotgalibacillus malaysiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.93
-
 4-nitrophenyl beta-D-glucopyranoside at pH 7.4 and 65°C Jeotgalibacillus malaysiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in sodium phosphate buffer Jeotgalibacillus malaysiensis

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 more than 85% activity at pH 6.0-7.5 Jeotgalibacillus malaysiensis