Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Colussi, F.; Da Silva, V.; Miller, I.; Cota, J.; De Oliveira, L.; De Oliveira Neto, M.; Squina, F.; Garcia, W.
    Oligomeric state and structural stability of two hyperthermophilic beta-glucosidases from Thermotoga petrophila (2015), Amino Acids, 47, 937-948 .
    View publication on PubMed
Search for more literature for 3.2.1.21

Application

Application Comment Organism
biofuel production biodegradation of lignocellulosic biomass involves a concerted attack by several enzymes, including beta-glucosidases as key component. Current methodologies for biomass conversion to biofuels employ physical and/or chemical pretreatments that disrupt the lignocellulosic biomass in plant cell walls in combination with enzymatic hydrolysis of the cellulose to produce free sugars. Thus, stable cellulolytic enzymes with high enzymatic activity in pretreatment biomass conditions, including high temperatures and acidic conditions, are essential at an industrial scale production. These two features makes beta-glucosidase TpBGL1 to be of significant biotechnological interest Thermotoga petrophila
biofuel production biodegradation of lignocellulosic biomass involves a concerted attack by several enzymes, including beta-glucosidases as key component. Current methodologies for biomass conversion to biofuels employ physical and/or chemical pretreatments that disrupt the lignocellulosic biomass in plant cell walls in combination with enzymatic hydrolysis of the cellulose to produce free sugars. Thus, stable cellulolytic enzymes with high enzymatic activity in pretreatment biomass conditions, including high temperatures and acidic conditions, are essential at an industrial scale production. These two features makes beta-glucosidase TpBGL3 to be of significant biotechnological interest Thermotoga petrophila

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermotoga petrophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 beta-glucosidase TpBGL1, SDS-PAGE Thermotoga petrophila
84000
-
 beta-glucosidase TpBGL3, SDS-PAGE Thermotoga petrophila
102000
-
 beta-glucosidase TpBGL3, dynamic light scattering at pH 6 Thermotoga petrophila
113000
-
 beta-glucosidase TpBGL1, dynamic light scattering at pH 4 Thermotoga petrophila
167000
-
 beta-glucosidase TpBGL1, dynamic light scattering at pH 4 Thermotoga petrophila
182000
-
 beta-glucosidase TpBGL3, dynamic light scattering at pH 6 Thermotoga petrophila

Organism

Organism UniProt Comment Textmining
Thermotoga petrophila A5IL43
-
-
Thermotoga petrophila A5IL97
-
-
Thermotoga petrophila DSM 13995 A5IL43
-
-
Thermotoga petrophila DSM 13995 A5IL97
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga petrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila DSM 13995 4-nitrophenol + D-glucose
-
 ?

Subunits

Subunits Comment Organism
homodimer beta-glucosidase TpBGL1 is a stable homodimer in solution. The oligomerization state does change in response to decreasing the pH from 6 to 4 at 20°C Thermotoga petrophila
homodimer beta-glucosidase TpBGL3 is a stable homodimer in solution. The oligomerization state does change in response to decreasing the pH from 6 to 4 at 20°C Thermotoga petrophila

Synonyms

Synonyms Comment Organism
TpBGL1
-
 Thermotoga petrophila
TpBGL3
-
 Thermotoga petrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Thermotoga petrophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 at pH 4 the ellipticity at 222 nm of beta-glucosidase TpBGL1 remains relatively constant at temperatures lower than 80°C. A progressive decrease is observed when the temperature is increased above 80°C Thermotoga petrophila
80 90 at temperatures between 80 and 90°C, beta-glucosidase TpBGL1 is more unstable at pH 4 than pH 6 Thermotoga petrophila
90
-
 for beta-glucosidase TpBGL3 at both pH 6 and 4, the ellipticities at 222 nm remain relatively constant at temperatures lower than 90°C. A decrease in the ellipticities at 222 nm is observed at temperatures higher than 90°C, suggesting a loss of regular secondary structure, the process is irreversible Thermotoga petrophila
100
-
 beta-glucosidase TpBGL1 at pH 6 can not be completely unfolded at 100°C. At pH 4 TpBGL1 drastically loses regular secondary structure at temperatures higher than 80°C with a complete unfolding at 100°C. At both pH values, the processes are essentially irreversible Thermotoga petrophila
100
-
 beta-glucosidase TpBGL3 can be thermally denatured at pH 4 and at pH 6, the process is irreversible Thermotoga petrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 beta-glucosidase TpBGL3 Thermotoga petrophila
6
-
 beta-glucosidase TpBGL1 Thermotoga petrophila

pH Range

pH Minimum pH Maximum Comment Organism
3 5 pH 3.0: about 65% of maximal activity, pH 5.0: about 70% of maximal activity, beta-glucosidase TpBGL3 Thermotoga petrophila
4 8 pH 4.0: about 45% of maximal activity, pH 8.0: about 60% of maximal activity, beta-glucosidase TpBGL1 Thermotoga petrophila

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 beta-glucosidase TpBGL1 shows higher stability at pH 6 than at pH 4 Thermotoga petrophila