Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | presence of 2 mM Zn2+ increases the relative activity of the immobilized enzyme to 192% and 199% with cellobiose and 4-nitrophenyl-beta-D-glucopyranoside as substrates | Thermotoga petrophila |
Application | Comment | Organism |
---|---|---|
industry | the enzyme is widely used in cellulose hydrolysis and the biological conversion and transformation of flavonoids and saponins. The properties of the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde, including thermal stability, pH stability, reusability, and tolerance of glucose, are greatly improved compared with free Tpebgl3. The coordinating metal cation Zn2+ enables the immobilized enzyme to exhibit higher catalytic efficiency. The addition of Zn2+ can greatly advance the reusability, thermostability, and glucose tolerance of immobilized enzyme, which is beneficial for industrial applications | Thermotoga petrophila |
synthesis | immobilization of enzyme on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde. The optimal conditions of immobilized enzyme are the same as that of the free enzyme, the highest activity with cellobiose as the substrate approaches 1.7 U/g. Immobilization improves the thermostability, pH stability and glucose tolerance, the residual activity is 68% of the initial activity at the end of 10 repeated cycles. 2 mM Zn2+ increases the relative activity of the immobilized enzyme to 192% and 199% with cellobiose and 4-nitrophenyl-beta-D-glucopyranoside as substrates, respectively and improves the reusability, high-temperature stability, and glucose tolerance | Thermotoga petrophila |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga petrophila |
General Stability | Organism |
---|---|
the residual activity of the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde is 68% of the initial activity at the end of 10 repeated cycles | Thermotoga petrophila |
Zn2+ greatly improves the reusability, glucose tolerance and high-temperature stability of the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde | Thermotoga petrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | when the glucose concentration is 400 mM, the native enzyme is completely inactivated, and the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde with 2mM Zn2+ maintains 63% of its initial activity, which is 19.5% higher than the activity of immobilized enzyme in the absence of Zn2+ | Thermotoga petrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.32 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, without Zn2+ | Thermotoga petrophila | |
3.03 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, 2 mM Zn2+ | Thermotoga petrophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 2 mM Zn2+ increases the relative activity of the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde to 192% and 199% with cellobiose and 4-nitrophenyl beta-D-glucopyranoside as substrates, respectively | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | - |
- |
- |
Thermotoga petrophila | A5IL43 | - |
- |
Thermotoga petrophila DSM 13995 | - |
- |
- |
Thermotoga petrophila DSM 13995 | A5IL43 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila DSM 13995 | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila | nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila DSM 13995 | nitrophenol + D-glucose | - |
? | |
cellobiose + H2O | - |
Thermotoga petrophila | 2 D-glucose | - |
? | |
cellobiose + H2O | the highest activity with cellobiose as the substrate approaches 1.7 U/g, native enzyme and enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde | Thermotoga petrophila | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Thermotoga petrophila DSM 13995 | 2 D-glucose | - |
? | |
cellobiose + H2O | the highest activity with cellobiose as the substrate approaches 1.7 U/g, native enzyme and enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde | Thermotoga petrophila DSM 13995 | 2 D-glucose | - |
? | |
cellulose + H2O | - |
Thermotoga petrophila | ? | - |
? | |
cellulose + H2O | - |
Thermotoga petrophila DSM 13995 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bgl3 | - |
Thermotoga petrophila |
Tpebgl3 | - |
Thermotoga petrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga petrophila |
90 | - |
optimal temperature of native enzyme and of the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde | Thermotoga petrophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 95 | 60°C: about 50% of maximal activity, 95°C: about 65% of maximal activity, native enzyme | Thermotoga petrophila |
60 | 95 | 60°C: about 50% of maximal activity, 95°C: about 75% of maximal activity, enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde | Thermotoga petrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
7 h, 84% of the residual activity of the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde is retained, 63% of the residual activity of the native enzyme is retained. The temperature stability of the immobilized enzyme is better than that of the free enzyme | Thermotoga petrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.89 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, without Zn2+ | Thermotoga petrophila | |
51.43 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, 2 mM Zn2+ | Thermotoga petrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermotoga petrophila |
5 | - |
optimal pH-value of native enzyme and of the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde | Thermotoga petrophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | pH 4.0: about 65% of maximal activity, pH 7.0: about 45% of maximal activity, enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde | Thermotoga petrophila |
4.5 | 6 | pH 4.5: about 85% of maximal activity, pH 6.0: about 65% of maximal activity, native enzyme | Thermotoga petrophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
42% of the initial activity for the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde remains, the activity of free enzyme is completely lost | Thermotoga petrophila |
8 | - |
the enzyme immobilized on macroporous resin NKA-9 modified with polyethylenimine and glutaraldehyde retains 79.6% of the initial activity underalkaline conditions (pH 8.0), which is higher by 32% compared with native enzyme | Thermotoga petrophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, without Zn2+ | Thermotoga petrophila | |
17 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 90°C, 2 mM Zn2+ | Thermotoga petrophila |