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Literature summary for 3.2.1.21 extracted from
- Purification, characterization and function analysis of an extracellular beta-glucosidase from elongating stipe cell walls in Coprinopsis cinerea (2016), FEMS Microbiol. Lett., 363, fnw078 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, 40% inhibition | Coprinopsis cinerea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
4-nitrophenyl beta-D-glucopyranoside | pH not specified in the publication, temperature not specified in the publication | Coprinopsis cinerea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Coprinopsis cinerea | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 5 mM, 110% of initial activity | Coprinopsis cinerea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 302200 | - |
PAGE | Coprinopsis cinerea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | A8NIX3 | - |
- |
| Coprinopsis cinerea ATCC MYA-4618 | A8NIX3 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Coprinopsis cinerea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Coprinopsis cinerea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stipe | stipe cell wall, enzyme is more highly expressed in the elongating apical stipe than in non-elongating basal regions | Coprinopsis cinerea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 117 | - |
substrate laminaritriose, pH not specified in the publication, temperature not specified in the publication | Coprinopsis cinerea |
| 129 | - |
substrate laminaritetraose, pH not specified in the publication, temperature not specified in the publication | Coprinopsis cinerea |
| 175 | - |
substrate cellobiose, pH not specified in the publication, temperature not specified in the publication | Coprinopsis cinerea |
| 212 | - |
substrate laminaribiose, pH not specified in the publication, temperature not specified in the publication | Coprinopsis cinerea |
| 268 | - |
substrate gentiobiose, pH not specified in the publication, temperature not specified in the publication | Coprinopsis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 laminaribiose | - |
Coprinopsis cinerea | D-glucose + laminaritriose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Coprinopsis cinerea | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Coprinopsis cinerea ATCC MYA-4618 | 4-nitrophenol + D-glucopyranose | - |
? | |
| cellobiose + H2O | - |
Coprinopsis cinerea | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Coprinopsis cinerea ATCC MYA-4618 | 2 D-glucose | - |
? | |
| gentiobiose + H2O | - |
Coprinopsis cinerea | D-glucose | - |
? | |
| gentiobiose + H2O | - |
Coprinopsis cinerea ATCC MYA-4618 | D-glucose | - |
? | |
| laminaribiose + H2O | - |
Coprinopsis cinerea | 2 D-glucose | - |
? | |
| laminaritetraose + 3 H2O | - |
Coprinopsis cinerea | 4 D-glucose | - |
? | |
| laminaritriose + 2 H2O | - |
Coprinopsis cinerea | 3 D-glucose | - |
? | |
| laminaritriose + 2 H2O | - |
Coprinopsis cinerea ATCC MYA-4618 | 3 D-glucose | - |
? | |
| additional information | the enzyme does not degrade beta-glucans with modified ends, whereas it hydrolyzes various beta-glucans with free ends and related oligosaccharides with beta-1,3-, beta-1,4- or beta-1,6-linkages. It shows glycosyltransferase activity, preferably transfering 1 glucose residue from the donor oligosaccharides to the acceptor oligosaccharides, yielding laminario-(n1) oligosaccharides and laminario-(n+1) oligosaccharides | Coprinopsis cinerea | ? | - |
? | |
| additional information | the enzyme does not degrade beta-glucans with modified ends, whereas it hydrolyzes various beta-glucans with free ends and related oligosaccharides with beta-1,3-, beta-1,4- or beta-1,6-linkages. It shows glycosyltransferase activity, preferably transfering 1 glucose residue from the donor oligosaccharides to the acceptor oligosaccharides, yielding laminario-(n1) oligosaccharides and laminario-(n+1) oligosaccharides | Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | 1 * 165200, plus 1 * 87900, plus 1 * 56800, SDS-PAGE | Coprinopsis cinerea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CC1G_08724 | - |
Coprinopsis cinerea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Coprinopsis cinerea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, 70% loss of activity | Coprinopsis cinerea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Coprinopsis cinerea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | stable for 30 min | Coprinopsis cinerea |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme causes a decrease in the molecular size of stipe wall beta-glucan by removing glucose and is more highly expressed in the elongating apical stipe than in non-elongating basal regions | Coprinopsis cinerea |
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