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Literature summary for 3.2.1.21 extracted from

  • Mallek-Fakhfakh, H.; Belghith, H.
    Physicochemical properties of thermotolerant extracellular beta-glucosidase from Talaromyces thermophilus and enzymatic synthesis of cello-oligosaccharides (2016), Carbohydr. Res., 419, 41-50 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-delta-lactone competitive Thermomyces dupontii
D-glucose competitive Thermomyces dupontii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Thermomyces dupontii
2.7
-
Salicin pH 5.0, 50°C Thermomyces dupontii
16.7
-
cellobiose pH 5.0, 50°C Thermomyces dupontii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomyces dupontii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
113000
-
gel filtration Thermomyces dupontii

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 10% v/v, almost no loss of activity Thermomyces dupontii

Organism

Organism UniProt Comment Textmining
Thermomyces dupontii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Thermomyces dupontii

Purification (Commentary)

Purification (Comment) Organism
-
Thermomyces dupontii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1429
-
pH 5.0, 50°C Thermomyces dupontii

Storage Stability

Storage Stability Organism
4°C, 24 h, 95% residual activity Thermomyces dupontii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermomyces dupontii 2-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermomyces dupontii 4-nitrophenol + D-glucose
-
?
amygdalin + 2 H2O 19% of the activity with 4-nitrophenyl-beta-D-glucopyranoside Thermomyces dupontii mandelonitrile + 2 beta-D-glucose
-
?
cellobiose + H2O 100% of the activity with 4-nitrophenyl-beta-D-glucopyranoside Thermomyces dupontii 2 D-glucose
-
?
esculin + H2O
-
Thermomyces dupontii beta-D-glucose + esculetin
-
?
maltose + H2O 80% of the activity with 4-nitrophenyl-beta-D-glucopyranoside Thermomyces dupontii 2 D-glucose
-
?
additional information enzyme hydrolyzes a variety of aryl/alkyl-beta-glucosides and disaccharides as well as (1->6) and (1->4)-beta-glucosidic linkages and alpha-glycosidic substrates Thermomyces dupontii ?
-
?
salicin + H2O 6% of the activity with 4-nitrophenyl-beta-D-glucopyranoside Thermomyces dupontii D-glucose + salicyl alcohol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 116000, SDS-PAGE Thermomyces dupontii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermomyces dupontii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 70 more than 80% of maximum activity Thermomyces dupontii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable for 34 h Thermomyces dupontii
60
-
1 h, 80% residual activity Thermomyces dupontii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Thermomyces dupontii

pH Range

pH Minimum pH Maximum Comment Organism
4
-
more than 80% of maximum activity Thermomyces dupontii
7
-
more than 80% of maximum activity Thermomyces dupontii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10
-
Thermomyces dupontii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
D-glucono-delta-lactone pH 5.0, 50°C Thermomyces dupontii
1.4
-
D-glucose pH 5.0, 50°C Thermomyces dupontii