Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucono-delta-lactone | competitive | Thermomyces dupontii | |
D-glucose | competitive | Thermomyces dupontii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 50°C | Thermomyces dupontii | |
2.7 | - |
Salicin | pH 5.0, 50°C | Thermomyces dupontii | |
16.7 | - |
cellobiose | pH 5.0, 50°C | Thermomyces dupontii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermomyces dupontii | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
113000 | - |
gel filtration | Thermomyces dupontii |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | 10% v/v, almost no loss of activity | Thermomyces dupontii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces dupontii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Thermomyces dupontii |
Purification (Comment) | Organism |
---|---|
- |
Thermomyces dupontii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1429 | - |
pH 5.0, 50°C | Thermomyces dupontii |
Storage Stability | Organism |
---|---|
4°C, 24 h, 95% residual activity | Thermomyces dupontii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermomyces dupontii | 2-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermomyces dupontii | 4-nitrophenol + D-glucose | - |
? | |
amygdalin + 2 H2O | 19% of the activity with 4-nitrophenyl-beta-D-glucopyranoside | Thermomyces dupontii | mandelonitrile + 2 beta-D-glucose | - |
? | |
cellobiose + H2O | 100% of the activity with 4-nitrophenyl-beta-D-glucopyranoside | Thermomyces dupontii | 2 D-glucose | - |
? | |
esculin + H2O | - |
Thermomyces dupontii | beta-D-glucose + esculetin | - |
? | |
maltose + H2O | 80% of the activity with 4-nitrophenyl-beta-D-glucopyranoside | Thermomyces dupontii | 2 D-glucose | - |
? | |
additional information | enzyme hydrolyzes a variety of aryl/alkyl-beta-glucosides and disaccharides as well as (1->6) and (1->4)-beta-glucosidic linkages and alpha-glycosidic substrates | Thermomyces dupontii | ? | - |
? | |
salicin + H2O | 6% of the activity with 4-nitrophenyl-beta-D-glucopyranoside | Thermomyces dupontii | D-glucose + salicyl alcohol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 116000, SDS-PAGE | Thermomyces dupontii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermomyces dupontii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 70 | more than 80% of maximum activity | Thermomyces dupontii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable for 34 h | Thermomyces dupontii |
60 | - |
1 h, 80% residual activity | Thermomyces dupontii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermomyces dupontii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | - |
more than 80% of maximum activity | Thermomyces dupontii |
7 | - |
more than 80% of maximum activity | Thermomyces dupontii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Thermomyces dupontii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
D-glucono-delta-lactone | pH 5.0, 50°C | Thermomyces dupontii | |
1.4 | - |
D-glucose | pH 5.0, 50°C | Thermomyces dupontii |