Application | Comment | Organism |
---|---|---|
degradation | saccharification of pretreated paddy straw by supplementing beta-glucosidase enzyme results in 1.34fold higher glucose release | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
molecular modeling of structure based on PDB entry 2XHY | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | 1 M, 70% residual activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, temperature not specified in the publication | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis RA10 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.9 | - |
pH 5.0, temperature not specified in the publication | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Bacillus subtilis | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Bacillus subtilis RA10 | 4-nitrophenol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57130, calculated, x * 57000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
78% of maximum activity | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
48 h, 68% residual activity | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, temperature not specified in the publication | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | - |
70% of maximum activity | Bacillus subtilis |