Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Borgi, I.; Gargouri, A.
    A novel high molecular weight thermo-acidoactive beta-glucosidase from Beauveria bassiana (2016), Appl. Biochem. Microbiol., 52, 602-607 .
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 3 mM, 48% residual activity Beauveria bassiana
Cu2+ 5 mM, 14% residual activity Beauveria bassiana
EDTA 10 mM, 90% residual activity Beauveria bassiana
Fe2+ 5 mM, 59% residual activity Beauveria bassiana
Mg2+ 5 mM, 92% residual activity Beauveria bassiana
Mn2+ 5 mM, 53% residual activity Beauveria bassiana
additional information not inhibitory: Co2+ Beauveria bassiana
Zn2+ 5 mM, 58% residual activity Beauveria bassiana

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Beauveria bassiana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 10 mM, 111% of initial activity Beauveria bassiana
K+ 2 M, 60% residual activity Beauveria bassiana
Na+ 2 M, 60% residual activity Beauveria bassiana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
400000 600000 PAGE Beauveria bassiana

Organism

Organism UniProt Comment Textmining
Beauveria bassiana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Beauveria bassiana 4-nitrophenol + beta-D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Beauveria bassiana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 24 h, 50% residual activity Beauveria bassiana
50
-
 1 h, 50% residual activity Beauveria bassiana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Beauveria bassiana

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 24 h, completely stable Beauveria bassiana

Expression

Organism Comment Expression
Beauveria bassiana glucose causes a repressor effect down
Beauveria bassiana expression in the presence of glucose, maltose, lactose, glycerol, starch, wheat bran and gruel. The highest level of beta-glucosidase activity is produced with wheat bran at the concentration of 3% up