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Literature summary for 3.2.1.21 extracted from

  • Souza, F.; Meleiro, L.; Machado, C.; Zimbardi, A.; Maldonado, R.; Souza, T.; Masui, D.; Murakami, M.; Jorge, J.; Ward, R.; Furriel, R.
    Gene cloning, expression and biochemical characterization of a glucose- and xylose-stimulated beta-glucosidase from Humicola insolens RP86 (2014), J. Mol. Catal. B, 106, 1-10 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 1.8fold stimulation at 50 mM Humicola insolens
D-xylose 1.9fold stimulation at 50 mM Humicola insolens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Humicola insolens

Inhibitors

Inhibitors Comment Organism Structure
L-arabinose 50 mM, 83% residual activity Humicola insolens
lactose 50 mM, 85% residual activity Humicola insolens
maltose 50 mM, 89% residual activity Humicola insolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
4-nitrophenyl beta-D-glucopyranoside presence of D-glucose, pH 6.0, 50°C Humicola insolens
0.38
-
cellobiose presence of D-glucose, pH 6.0, 50°C Humicola insolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56100
-
dynamic light scattering Humicola insolens

Organism

Organism UniProt Comment Textmining
Humicola insolens A0A076JRL8
-
-
Humicola insolens RP86 A0A076JRL8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
substrate 4-nitrophenyl beta-D-xylopyranoside, presence of D-glucose, pH 6.0, 50°C Humicola insolens
10.6
-
substrate salicin, presence of D-glucose, pH 6.0, 50°C Humicola insolens
16.8
-
substrate lactose, presence of D-glucose, pH 6.0, 50°C Humicola insolens
19.9
-
substrate 4-nitrophenyl beta-D-galactopyranoside, presence of D-glucose, pH 6.0, 50°C Humicola insolens
36.4
-
substrate 4-nitrophenyl beta-D-glucopyranoside, presence of D-glucose, pH 6.0, 50°C Humicola insolens
183.4
-
substrate cellobiose, presence of D-glucose, pH 6.0, 50°C Humicola insolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Humicola insolens 4-nitrophenyl + D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Humicola insolens 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Humicola insolens RP86 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Humicola insolens 4-nitrophenol + D-xylose
-
?
cellobiose + H2O
-
Humicola insolens 2 D-glucose
-
?
cellobiose + H2O
-
Humicola insolens RP86 2 D-glucose
-
?
lactose + H2O
-
Humicola insolens D-glucose + D-galactose
-
?
lactose + H2O
-
Humicola insolens RP86 D-glucose + D-galactose
-
?
additional information broad substrate specificity, specific for substrates having beta-glycosidic linkages, with strong preference for glucose, followed by galactose, at the non-reducing end. No activity with sucrose, maltose and trehalose, carboxymethyl cellulose and avicel Humicola insolens ?
-
?
additional information broad substrate specificity, specific for substrates having beta-glycosidic linkages, with strong preference for glucose, followed by galactose, at the non-reducing end. No activity with sucrose, maltose and trehalose, carboxymethyl cellulose and avicel Humicola insolens RP86 ?
-
?
salicin + H2O
-
Humicola insolens D-glucose + salicyl alcohol
-
?
salicin + H2O
-
Humicola insolens RP86 D-glucose + salicyl alcohol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 54100, calulated, 1 * 59000, SDS-PAGE of recombinant His-tagged protein Humicola insolens

Synonyms

Synonyms Comment Organism
Bglhi
-
Humicola insolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Humicola insolens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable for 1 h Humicola insolens
55
-
half-life 50 min Humicola insolens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.9
-
4-nitrophenyl beta-D-glucopyranoside presence of D-glucose, pH 6.0, 50°C Humicola insolens
172.1
-
cellobiose presence of D-glucose, pH 6.0, 50°C Humicola insolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7
-
Humicola insolens

pH Range

pH Minimum pH Maximum Comment Organism
3
-
70% residual activity Humicola insolens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 8
-
Humicola insolens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
169
-
4-nitrophenyl beta-D-glucopyranoside presence of D-glucose, pH 6.0, 50°C Humicola insolens
453
-
cellobiose presence of D-glucose, pH 6.0, 50°C Humicola insolens