Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-delta-gluconolactone | competitive | Thermoascus aurantiacus | |
D-glucose | competitive | Thermoascus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.114 | - |
4-nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
0.21 | - |
gentiobiose | pH 5, 50°C | Thermoascus aurantiacus | |
0.25 | - |
2-Nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
0.272 | - |
n-octyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
0.434 | - |
beta-beta-trehalose | pH 5, 50°C | Thermoascus aurantiacus | |
0.637 | - |
cellobiose | pH 5, 50°C | Thermoascus aurantiacus | |
1.34 | - |
sophorose | pH 5, 50°C | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
350000 | - |
gel filtration | Thermoascus aurantiacus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | activation below 50%, inhibitory above | Thermoascus aurantiacus |
isopropanol | activation below 50%, inhibitory above | Thermoascus aurantiacus |
Methanol | activation below 50%, inhibitory above | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Thermoascus aurantiacus IMI 216529 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoascus aurantiacus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus | 2-nitrophenol + D-glucose | - |
? | |
2-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus IMI 216529 | 2-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus IMI 216529 | 4-nitrophenol + D-glucose | - |
? | |
beta-beta-trehalose + H2O | - |
Thermoascus aurantiacus | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Thermoascus aurantiacus | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Thermoascus aurantiacus IMI 216529 | 2 D-glucose | - |
? | |
gentiobiose + H2O | - |
Thermoascus aurantiacus | 2 D-glucose | - |
? | |
additional information | enzyme hydrolyses aryl and alkyl beta-D-glucosides and cello-oligosaccharides, and is specific for substrates with a beta-glycosidic linkage. The hydroxy groups at positions 2, 4 and 6 of a glucose residue at the non-reducing end of a disaccharide are essential for catalysis. Enzyme releases one glucose unit at a time from the non-reducing end of cello-oligosaccharides, and the rate of hydrolysis decreases with an increase in chain length. The enzyme catalyses the synthesis of methyl, ethyl and propyl beta-D-glucosides in the presence of methanol, ethanol and propan-2-ol respectively with either glucose or cellobiose, cellobiose is preferred | Thermoascus aurantiacus | ? | - |
? | |
additional information | enzyme hydrolyses aryl and alkyl beta-D-glucosides and cello-oligosaccharides, and is specific for substrates with a beta-glycosidic linkage. The hydroxy groups at positions 2, 4 and 6 of a glucose residue at the non-reducing end of a disaccharide are essential for catalysis. Enzyme releases one glucose unit at a time from the non-reducing end of cello-oligosaccharides, and the rate of hydrolysis decreases with an increase in chain length. The enzyme catalyses the synthesis of methyl, ethyl and propyl beta-D-glucosides in the presence of methanol, ethanol and propan-2-ol respectively with either glucose or cellobiose, cellobiose is preferred | Thermoascus aurantiacus IMI 216529 | ? | - |
? | |
n-octyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus | n-octanol + D-glucose | - |
? | |
sophorose + H2O | - |
Thermoascus aurantiacus | 2 D-glucose | - |
? | |
sophorose + H2O | - |
Thermoascus aurantiacus IMI 216529 | 2 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 120000, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermoascus aurantiacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
70% of maximum activity | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable for 48 h | Thermoascus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
136 | - |
sophorose | pH 5, 50°C | Thermoascus aurantiacus | |
141 | - |
gentiobiose | pH 5, 50°C | Thermoascus aurantiacus | |
235 | - |
2-Nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
235 | - |
n-octyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
242 | - |
4-nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
284 | - |
cellobiose | pH 5, 50°C | Thermoascus aurantiacus | |
284 | - |
beta-beta-trehalose | pH 5, 50°C | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Thermoascus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.8 | 5 | - |
Thermoascus aurantiacus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
stable for 48 h | Thermoascus aurantiacus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
D-delta-gluconolactone | pH 5, 50°C | Thermoascus aurantiacus | |
0.29 | - |
D-glucose | pH 5, 50°C | Thermoascus aurantiacus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
102 | - |
sophorose | pH 5, 50°C | Thermoascus aurantiacus | |
446 | - |
cellobiose | pH 5, 50°C | Thermoascus aurantiacus | |
655 | - |
beta-beta-trehalose | pH 5, 50°C | Thermoascus aurantiacus | |
684 | - |
gentiobiose | pH 5, 50°C | Thermoascus aurantiacus | |
864 | - |
n-octyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
940 | - |
2-Nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus | |
2130 | - |
4-nitrophenyl beta-D-glucoside | pH 5, 50°C | Thermoascus aurantiacus |