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Literature summary for 3.2.1.21 extracted from

  • Anand L.; Vithayathil P.J.
    Purification and properties of beta glucosidase from thermophilic fungus humicola lanuginosa griffon and maublanc bunce (1989), J. Ferment. Bioeng., 67, 380-386.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
cellobiose pH and temperature not specified in the publication Thermomyces lanuginosus
0.5
-
4-nitrophenyl beta-D-glucopyranoside pH and temperature not specified in the publication Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomyces lanuginosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
gel filtration Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 9% carbohydrate Thermomyces lanuginosus

Purification (Commentary)

Purification (Comment) Organism
-
Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermomyces lanuginosus 4-nitrophenol + D-glucopyranose
-
?
cellobiose + H2O
-
Thermomyces lanuginosus 2 D-glucose
-
?
additional information the enzyme is capable of hydrolysing larchwood xylan, xylobiose and 4-nitrophenyl-beta-D-xyloside, though to a lesser extent. The enzyme was specific for the beta-configuration and glucose moiety in the substrate Thermomyces lanuginosus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 25°C, stable for 24 h Thermomyces lanuginosus