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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of a beta-glucosidase from solid-state cultures of Humicola grisea var. thermoidea (1996), Can. J. Microbiol., 42, 1-5.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Trichocladium griseum |  |
| D-gluconic acid lactone | - |
Trichocladium griseum | |
| D-glucose | - |
Trichocladium griseum | |
| Hg2+ | - |
Trichocladium griseum | |
| Mn2+ | - |
Trichocladium griseum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.316 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication | Trichocladium griseum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
2 * 82000, SDS-PAGE | Trichocladium griseum |
| 156000 | - |
gel filtration | Trichocladium griseum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichocladium griseum | - |
var. thermoidea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trichocladium griseum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichocladium griseum | 4-nitrophenol + D-glucopyranose | - |
? | |
| cellobiose + H2O | - |
Trichocladium griseum | 2 D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 82000, SDS-PAGE | Trichocladium griseum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Trichocladium griseum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 h, stable | Trichocladium griseum |
| 65 | - |
half-life: 15 min | Trichocladium griseum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | - |
Trichocladium griseum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
D-glucose | pH and temperature not specified in the publication | Trichocladium griseum | |
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