Cloned (Comment) | Organism |
---|---|
gene cba3, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of the extracellular His6-tagged enzyme in Escherichia coli strain JM101, transformants expressing recombinant Cba3 grow readily in minimal media using cellobiose as the sole carbon source | Cellulomonas biazotea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of inactive Cba3 deletants lacking either the N-terminal 10 amino acids or the C-terminal 10 residues | Cellulomonas biazotea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | despite lacking a classical N-terminal signal peptide, the enzyme is a secretory protein | Cellulomonas biazotea | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Cellulomonas biazotea | - |
2 beta-D-glucose | - |
? | |
cellobiose + H2O | Cellulomonas biazotea ATCC 486 | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas biazotea | G1C1T5 | gene cba3 | - |
Cellulomonas biazotea ATCC 486 | G1C1T5 | gene cba3 | - |
Purification (Comment) | Organism |
---|---|
recombinant extracellular His6-tagged enzyme from Escherichia coli strain JM101 culture supernatant by cobalt affinity chromatography, cleavage of the fusion protein with thrombin | Cellulomonas biazotea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Cellulomonas biazotea | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Cellulomonas biazotea ATCC 486 | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Cellulomonas biazotea | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Cellulomonas biazotea ATCC 486 | 2 beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bgl | - |
Cellulomonas biazotea |
Cba3 | - |
Cellulomonas biazotea |
cellobiase | - |
Cellulomonas biazotea |
GH1 beta-glucosidase | - |
Cellulomonas biazotea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cellulomonas biazotea |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 1, GH1 | Cellulomonas biazotea |
malfunction | enzyme deletion mutants lacking either the N-terminal 10 amino acids or the C-terminal 10 residues are biologically inactive | Cellulomonas biazotea |