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Literature summary for 3.2.1.21 extracted from

  • Lee, K.W.; Han, N.S.; Kim, J.H.
    Purification and characterization of beta-glucosidase from Weissella cibaria 37 (2012), J. Microbiol. Biotechnol., 22, 1705-1713.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Weissella cibaria

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM Cu2+ reduces the activity to 46.1% Weissella cibaria
E-64 1 mM E-64 reduces the activity to 78.3% Weissella cibaria
Fe3+ 5 mM Fe3+ reduces the activity to 55.8% Weissella cibaria
Mn2+ 5 mM Mn2+ reduces the activity to 86.9% Weissella cibaria
additional information not inhibited by EDTA and phenylmethylsulfonyl fluoride Weissella cibaria
pepstatin 1 mM pepstatin reduces the activity to 45.2% Weissella cibaria
SDS 1 mM SDS reduces the activity to 16.9% Weissella cibaria
Zn2+ 5 mM Cu2+ reduces the activity to 56.7% Weissella cibaria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.04
-
 4-nitrophenyl beta-D-glucopyranoside at pH 5.5 and 45°C Weissella cibaria

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM Ca2+ slightly increases the activity to 108.2% Weissella cibaria
additional information not influenced by Co2+, K+, and Mg2+ Weissella cibaria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49951
-
 x * 49951, calculated from amino acid sequence Weissella cibaria
50000
-
 x * 50000, SDS-PAGE Weissella cibaria

Organism

Organism UniProt Comment Textmining
Weissella cibaria I6TNE2
-
-
Weissella cibaria 37 I6TNE2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni+2-nitrilotriacetic acid column chromatography Weissella cibaria

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Weissella cibaria 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Weissella cibaria 37 4-nitrophenol + D-glucopyranose
-
 ?
carboxymethyl cellulose + H2O
-
 Weissella cibaria ?
-
 ?
carboxymethyl cellulose + H2O
-
 Weissella cibaria 37 ?
-
 ?
cellobiose + H2O
-
 Weissella cibaria ?
-
 ?
cellobiose + H2O
-
 Weissella cibaria 37 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Weissella cibaria
? x * 49951, calculated from amino acid sequence Weissella cibaria

Synonyms

Synonyms Comment Organism
beta-D-glucoside glucohydrolase
-
 Weissella cibaria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Weissella cibaria

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 more than 50% activity between 30 and 52°C. Activity decreases rapidly at temperature above 55°C Weissella cibaria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 enzyme activity decreases rapidly during the first 10 min and then gradually decreases to about 30% until 60 min when incubated at 40-50°C Weissella cibaria

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30.63
-
 4-nitrophenyl beta-D-glucopyranoside at pH 5.5 and 45°C Weissella cibaria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Weissella cibaria

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 more than 50% activity between pH 5.0 and 7.5 Weissella cibaria

pI Value

Organism Comment pI Value Maximum pI Value
Weissella cibaria calculated from amino acid sequence
-
 5.1