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Literature summary for 3.2.1.21 extracted from

  • Synowiecki, J.; Wolosowska, S.
    Immobilization of thermostable beta-glucosidase from Sulfolobus shibatae by cross-linking with transglutaminase (2006), Enzyme Microb. Technol., 39, 1417-1422.
No PubMed abstract available

Application

Application Comment Organism
nutrition properties of the thermostable beta-glucosidase from Sulfolobus shibatae immobilized on silica gel by cross-linking with transglutaminase indicate possible suitability of this preparation for hydrolysis of lactose during milk and whey processing. Due to high thermal stability the immobilized enzyme can be used at temperatures, which restrict microbial growth during a long operating time of continuous-flow reactor Saccharolobus shibatae

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA Saccharolobus shibatae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Saccharolobus shibatae

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae
-
-
-
Saccharolobus shibatae DSM 5389
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0039
-
substrate: 2-nitrophenyl-beta-D-galactopyranoside, 70°C, pH 5.5 Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus shibatae 2-nitrophenol + beta-D-galactose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus shibatae DSM 5389 2-nitrophenol + beta-D-galactose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
98
-
immobilized and soluble enzyme, substrate: 2-nitrophenyl-beta-D-galactopyranoside Saccharolobus shibatae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
pH 5.5, 1 h, immobilized enzyme, no loss of activity in absence of substrate Saccharolobus shibatae
90
-
pH 5.5, 1 h, immobilized enzyme, 30% loss of activity in absence of substrate Saccharolobus shibatae
90
-
pH 5.5, 1 h, immobilized enzyme, 72% loss of activity in absence of substrate Saccharolobus shibatae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
enzyme immobilized at pH 5.0 Saccharolobus shibatae