Application | Comment | Organism |
---|---|---|
nutrition | properties of the thermostable beta-glucosidase from Sulfolobus shibatae immobilized on silica gel by cross-linking with transglutaminase indicate possible suitability of this preparation for hydrolysis of lactose during milk and whey processing. Due to high thermal stability the immobilized enzyme can be used at temperatures, which restrict microbial growth during a long operating time of continuous-flow reactor | Saccharolobus shibatae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by EDTA | Saccharolobus shibatae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | - |
- |
- |
Saccharolobus shibatae DSM 5389 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0039 | - |
substrate: 2-nitrophenyl-beta-D-galactopyranoside, 70°C, pH 5.5 | Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus shibatae | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus shibatae DSM 5389 | 2-nitrophenol + beta-D-galactose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
immobilized and soluble enzyme, substrate: 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus shibatae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
pH 5.5, 1 h, immobilized enzyme, no loss of activity in absence of substrate | Saccharolobus shibatae |
90 | - |
pH 5.5, 1 h, immobilized enzyme, 30% loss of activity in absence of substrate | Saccharolobus shibatae |
90 | - |
pH 5.5, 1 h, immobilized enzyme, 72% loss of activity in absence of substrate | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
enzyme immobilized at pH 5.0 | Saccharolobus shibatae |