Literature summary for 3.2.1.21 extracted from

Naz, S.; Ikram, N.; Rajoka, M.I.; Sadaf, S.; Akhtar, M.W.
Enhanced production and characterization of a beta-glucosidase from Bacillus halodurans expressed in Escherichia coli (2010), Biochemistry, 75, 513-525.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli under regulation of a T7/lac promoter. On induction with isopropyl-beta-D-1-thiogalactopyranoside, the enzyme expresses at 40% of the cell protein producing 238 mg/liter culture. With increase in culture cell density to A600 12 in auto-inducing M9NG medium, beta-glucosidase production increases 3fold. Approximately 70% of the expressed enzyme is in a soluble form, while the rest is in an insoluble fraction of the cell lysate	Halalkalibacterium halodurans

Cloned (Comment)

Organism

expressed in Escherichia coli under regulation of a T7/lac promoter. On induction with isopropyl-beta-D-1-thiogalactopyranoside, the enzyme expresses at 40% of the cell protein producing 238 mg/liter culture. With increase in culture cell density to A600 12 in auto-inducing M9NG medium, beta-glucosidase production increases 3fold. Approximately 70% of the expressed enzyme is in a soluble form, while the rest is in an insoluble fraction of the cell lysate

Halalkalibacterium halodurans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4	-	4-nitrophenyl-beta-D-glucopyranoside	pH 8.0, 45°C	Halalkalibacterium halodurans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51600	-	MALDI-TOF	Halalkalibacterium halodurans

Organism

Organism	UniProt	Comment	Textmining
Halalkalibacterium halodurans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using ammonium sulfate precipitation followed by ion-exchange chromatography	Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Halalkalibacterium halodurans	4-nitrophenol + D-glucopyranose	-	?

Synonyms

Synonyms	Comment	Organism
beta-glucosidase	-	Halalkalibacterium halodurans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Halalkalibacterium halodurans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.75	-	4-nitrophenyl-beta-D-glucopyranoside	pH 8.0, 45°C	Halalkalibacterium halodurans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Halalkalibacterium halodurans