Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoic acid | 56.34% inhibitory effect at 1 mM | Rhynchophorus palmarum | |
5,5'-dithio-bis(2-nitrobenzoic acid) | 8.67% inhibitory effect at 1 mM | Rhynchophorus palmarum | |
Cu2+ | 13.64% inhibitory effect at 1 mM | Rhynchophorus palmarum | |
Fe3+ | 71.97% inhibitory effect at 1 mM | Rhynchophorus palmarum | |
additional information | the enzyme is not affected by Ba2+, Mg2+, Mn2+, Sr2+, and Ca2+ | Rhynchophorus palmarum | |
Zn2+ | 67.06% inhibitory effect at 1 mM | Rhynchophorus palmarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
1 * 58000, SDS-PAGE | Rhynchophorus palmarum |
60000 | - |
gel filtration | Rhynchophorus palmarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhynchophorus palmarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography, ammonium sulfate precipitation, Sephacryl S-100 gel filtration, and phenyl-Sepharose column chromatography | Rhynchophorus palmarum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive fluid | - |
Rhynchophorus palmarum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.69 | - |
crude extract, at 37°C | Rhynchophorus palmarum |
25.1 | - |
after 36.38fold purification, at 37°C | Rhynchophorus palmarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethanol + cellobiose | beta-glucosidase is capable of catalyzing transglycosylation reactions | Rhynchophorus palmarum | ? | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Rhynchophorus palmarum | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Rhynchophorus palmarum | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Rhynchophorus palmarum | 2 beta-D-glucose | - |
? | |
cellodextrin + H2O | - |
Rhynchophorus palmarum | ? | - |
? | |
additional information | beta-glucosidase requires strictly beta-gluco configuration for activity, it cleaves glucose-glucose beta-(1-4) linkages better than beta-(1-2), and beta-(1-6) linkages | Rhynchophorus palmarum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Rhynchophorus palmarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rhynchophorus palmarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 55 | the purified beta-glucosidase is stable for 100 min at 37°C, but unstable at 55°C with half-life of 20 min, complete loss of activity occurs after 120 min at 55°C | Rhynchophorus palmarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
pH-optimum for hydrolysis reactions | Rhynchophorus palmarum |
6.6 | - |
pH-optimum for transglucosylation reactions | Rhynchophorus palmarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 7 | maximum transglucosylation pH range | Rhynchophorus palmarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | the enzyme is stable from pH 5.0-6.0 for 120 min at 37°C | Rhynchophorus palmarum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
134.9 | - |
cellobiose | in 100 mM acetate buffer, pH 5.0, at 37°C | Rhynchophorus palmarum | |
240.5 | - |
4-nitrophenyl-beta-D-glucoside | in 100 mM acetate buffer, pH 5.0, at 37°C | Rhynchophorus palmarum |