Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Spodoptera frugiperda |
Protein Variants | Comment | Organism |
---|---|---|
E451A | the double mutant shows decreased activity compared to the wild type enzyme | Spodoptera frugiperda |
E451S | the double mutant shows decreased activity compared to the wild type enzyme | Spodoptera frugiperda |
Q39E | the double mutant shows decreased activity compared to the wild type enzyme | Spodoptera frugiperda |
Q39E/E451A | the double mutant shows decreased activity compared to the wild type enzyme | Spodoptera frugiperda |
Q39E/E451S | the double mutant shows decreased activity compared to the wild type enzyme | Spodoptera frugiperda |
Q39N | the double mutant shows decreased activity compared to the wild type enzyme | Spodoptera frugiperda |
Q39N/E451S | the double mutant shows decreased activity compared to the wild type enzyme | Spodoptera frugiperda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39N/E451S, at 30°C | Spodoptera frugiperda | |
0.22 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E/E451A, at 30°C | Spodoptera frugiperda | |
0.26 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39N, at 30°C | Spodoptera frugiperda | |
0.38 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E/E451S, at 30°C | Spodoptera frugiperda | |
0.49 | - |
4-nitrophenyl-beta-D-fucoside | wild type enzyme, at 30°C | Spodoptera frugiperda | |
0.5 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme E451A, at 30°C | Spodoptera frugiperda | |
0.68 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme E451S, at 30°C | Spodoptera frugiperda | |
1.5 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39N, at 30°C | Spodoptera frugiperda | |
1.53 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme E451A, at 30°C | Spodoptera frugiperda | |
1.7 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme E451S, at 30°C | Spodoptera frugiperda | |
2 | - |
4-nitrophenyl-beta-D-galactoside | wild type enzyme, at 30°C | Spodoptera frugiperda | |
2.2 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39N/E451S, at 30°C | Spodoptera frugiperda | |
2.3 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E, at 30°C | Spodoptera frugiperda | |
2.3 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E/E451S, at 30°C | Spodoptera frugiperda | |
2.7 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E/E451A, at 30°C | Spodoptera frugiperda | |
2.8 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E, at 30°C | Spodoptera frugiperda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Spodoptera frugiperda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-fucoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
betagly | - |
Spodoptera frugiperda |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000285 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme E451A, at 30°C | Spodoptera frugiperda | |
0.00038 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39N, at 30°C | Spodoptera frugiperda | |
0.0004 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39N/E451S, at 30°C | Spodoptera frugiperda | |
0.00053 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme E451S, at 30°C | Spodoptera frugiperda | |
0.0006 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39N/E451S, at 30°C | Spodoptera frugiperda | |
0.00061 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme E451A, at 30°C | Spodoptera frugiperda | |
0.00093 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme E451S, at 30°C | Spodoptera frugiperda | |
0.0036 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E/E451A, at 30°C | Spodoptera frugiperda | |
0.0065 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E/E451S, at 30°C | Spodoptera frugiperda | |
0.022 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39N, at 30°C | Spodoptera frugiperda | |
0.0236 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E/E451A, at 30°C | Spodoptera frugiperda | |
0.0342 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E/E451S, at 30°C | Spodoptera frugiperda | |
0.0377 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E, at 30°C | Spodoptera frugiperda | |
0.35 | - |
4-nitrophenyl-beta-D-galactoside | wild type enzyme, at 30°C | Spodoptera frugiperda | |
1.49 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E, at 30°C | Spodoptera frugiperda | |
3.3 | - |
4-nitrophenyl-beta-D-fucoside | wild type enzyme, at 30°C | Spodoptera frugiperda |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39N/E451S, at 30°C | Spodoptera frugiperda | |
0.00025 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39N, at 30°C | Spodoptera frugiperda | |
0.00053 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme E451S, at 30°C | Spodoptera frugiperda | |
0.00057 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme E451A, at 30°C | Spodoptera frugiperda | |
0.0039 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme E451A, at 30°C | Spodoptera frugiperda | |
0.005 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39N/E451S, at 30°C | Spodoptera frugiperda | |
0.0087 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E/E451A, at 30°C | Spodoptera frugiperda | |
0.0134 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E, at 30°C | Spodoptera frugiperda | |
0.014 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E/E451S, at 30°C | Spodoptera frugiperda | |
0.016 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E/E451A, at 30°C | Spodoptera frugiperda | |
0.017 | - |
4-nitrophenyl-beta-D-galactoside | mutant enzyme Q39E/E451S, at 30°C | Spodoptera frugiperda | |
0.027 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme E451S, at 30°C | Spodoptera frugiperda | |
0.084 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39N, at 30°C | Spodoptera frugiperda | |
0.17 | - |
4-nitrophenyl-beta-D-galactoside | wild type enzyme, at 30°C | Spodoptera frugiperda | |
0.64 | - |
4-nitrophenyl-beta-D-fucoside | mutant enzyme Q39E, at 30°C | Spodoptera frugiperda | |
6.7 | - |
4-nitrophenyl-beta-D-fucoside | wild type enzyme, at 30°C | Spodoptera frugiperda |