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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of a beta-glucosidase from the root parasitic plant Orobanche minor Sm. (2010), Biosci. Biotechnol. Biochem., 74, 646-648.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Orobanche minor |  |
| cellobiose | complete inhibition at 1 mM | Orobanche minor | |
| D-glucose | complete inhibition at 1 mM | Orobanche minor | |
| Hg2+ | - |
Orobanche minor | |
| Monoiodoacetic acid | - |
Orobanche minor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
Salicin | - |
Orobanche minor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates activity | Orobanche minor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
estimated from SDS-PAGE | Orobanche minor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Orobanche minor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CM-Sephadex C-50 gel filtration, DEAE-Sephadex A-50 gel filtration, Sephadex G-200 gel filtration, and Con A Sepharose column chromatography | Orobanche minor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Orobanche minor | - |
| root tubercle | - |
Orobanche minor | - |
| shoot | - |
Orobanche minor | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.07 | - |
crude extract | Orobanche minor |
| 21.3 | - |
purified enzyme | Orobanche minor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Orobanche minor | 4-nitrophenol + D-glucopyranose | - |
? | |
| additional information | does not hydrolyze the cell wall of Orobanche minor or cellohexaose | Orobanche minor | ? | - |
? | |
| salicin + H2O | - |
Orobanche minor | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Orobanche minor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the enzyme is stable up to 50°C | Orobanche minor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Orobanche minor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | stable between pH 4.0 and 10.0 at 4°C | Orobanche minor |
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