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Literature summary for 3.2.1.21 extracted from

  • Feng, Y.; Duan, C.J.; Liu, L.; Tang, J.L.; Feng, J.X.
    Properties of a metagenome-derived beta-glucosidase from the contents of rabbit cecum (2009), Biosci. Biotechnol. Biochem., 73, 1470-1473.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

Protein Variants

Protein Variants Comment Organism
D772A the mutant shows less than 1/10000 activity of the wild type enzyme uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
Cr3+ 42% residual activity at 5 mM uncultured bacterium
Cu2+ 86% residual activity at 5 mM uncultured bacterium
EDTA effective inhibitor, 32% residual activity at 5 mM uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 124% relative activity at 5 mM uncultured bacterium
Co2+ 129% relative activity at 5 mM uncultured bacterium
Fe2+ 107% relative activity at 5 mM uncultured bacterium
Mg2+ 106% relative activity at 5 mM uncultured bacterium
Mn2+ 108% relative activity at 5 mM uncultured bacterium
additional information not stimulated by Zn2+, Li+, Fe3+, and K+ uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
SDS-PAGE uncultured bacterium
101000
-
calculated from amino acid sequence uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium Q3HXC2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Mono Q column chromatography uncultured bacterium

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.91
-
crude extract uncultured bacterium
46.3
-
purified enzyme uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O low activity uncultured bacterium 4-nitrophenol + cellobiose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
uncultured bacterium 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity uncultured bacterium 4-nitrophenol + beta-D-xylopyranose
-
?
cellobiose + H2O low activity uncultured bacterium 2 beta-D-glucose
-
?
cellotetraose + 3 H2O low activity uncultured bacterium 4 beta-D-glucose
-
?
cellotriose + H2O low activity uncultured bacterium 3 D-glucose
-
?
additional information no hydrolysis of xylan, cellopentaose, cellohexaose, and carboxymethylcellulose uncultured bacterium ?
-
?

Synonyms

Synonyms Comment Organism
Umbgl3B
-
uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 50 the enzyme is stable from 10-30°C, about 75% activity at 40°C, about 30% activity at 50°C, almost inactive at 50°C uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
uncultured bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 about 60% activity at pH 3.0, about 80% activity at pH 4.0, the enzyme is stable at pH 5.0-9.0, activity drops sharply at pH 10.0 uncultured bacterium