Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | uncultured bacterium |
Protein Variants | Comment | Organism |
---|---|---|
D772A | the mutant shows less than 1/10000 activity of the wild type enzyme | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | 42% residual activity at 5 mM | uncultured bacterium | |
Cu2+ | 86% residual activity at 5 mM | uncultured bacterium | |
EDTA | effective inhibitor, 32% residual activity at 5 mM | uncultured bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 124% relative activity at 5 mM | uncultured bacterium | |
Co2+ | 129% relative activity at 5 mM | uncultured bacterium | |
Fe2+ | 107% relative activity at 5 mM | uncultured bacterium | |
Mg2+ | 106% relative activity at 5 mM | uncultured bacterium | |
Mn2+ | 108% relative activity at 5 mM | uncultured bacterium | |
additional information | not stimulated by Zn2+, Li+, Fe3+, and K+ | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
SDS-PAGE | uncultured bacterium |
101000 | - |
calculated from amino acid sequence | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | Q3HXC2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Mono Q column chromatography | uncultured bacterium |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.91 | - |
crude extract | uncultured bacterium |
46.3 | - |
purified enzyme | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | low activity | uncultured bacterium | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
uncultured bacterium | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | uncultured bacterium | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
cellobiose + H2O | low activity | uncultured bacterium | 2 beta-D-glucose | - |
? | |
cellotetraose + 3 H2O | low activity | uncultured bacterium | 4 beta-D-glucose | - |
? | |
cellotriose + H2O | low activity | uncultured bacterium | 3 D-glucose | - |
? | |
additional information | no hydrolysis of xylan, cellopentaose, cellohexaose, and carboxymethylcellulose | uncultured bacterium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Umbgl3B | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | the enzyme is stable from 10-30°C, about 75% activity at 40°C, about 30% activity at 50°C, almost inactive at 50°C | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
uncultured bacterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | about 60% activity at pH 3.0, about 80% activity at pH 4.0, the enzyme is stable at pH 5.0-9.0, activity drops sharply at pH 10.0 | uncultured bacterium |