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Literature summary for 3.2.1.21 extracted from

  • Pal, S.; Banik, S.P.; Ghorai, S.; Chowdhury, S.; Khowala, S.
    Purification and characterization of a thermostable intra-cellular beta-glucosidase with transglycosylation properties from filamentous fungus Termitomyces clypeatus (2010), Biores. Technol., 101, 2412-2420.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol the purified enzyme shows 152.9% relative activity at 1% (v/v) Termitomyces clypeatus
dithiothreitol the purified enzyme shows 108.4% relative activity at 20 mM Termitomyces clypeatus

Application

Application Comment Organism
synthesis the enzyme is useful in synthetic biology to produce complex bioactive glycosides and to avoid chemical hazards Termitomyces clypeatus

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-D-glucose
-
 Termitomyces clypeatus
beta-mercaptoethanol the mycelial extract shows 87.1% residual activity at 1% (v/v) Termitomyces clypeatus
Ca2+ the mycelial extract shows 59.5% residual activity at 20 mM, the purified enzyme shows 53.5% residual activity at 20 mM Termitomyces clypeatus
Cu2+ the mycelial extract shows 58.1% residual activity at 2 mM, the purified enzyme shows 44.4% residual activity at 2 mM Termitomyces clypeatus
dithiothreitol the mycelial extract shows 75.7% residual activity at 20 mM Termitomyces clypeatus
EDTA the mycelial extract shows 64.3% residual activity at 20 mM, the purified enzyme shows 37.4% residual activity at 20 mM Termitomyces clypeatus
Hg2+ the mycelial extract shows 26.8% residual activity at 20 mM, the purified enzyme shows 1.2% residual activity at 20 mM Termitomyces clypeatus
K+ the mycelial extract shows 70.9% residual activity at 20 mM, the purified enzyme shows 41.5% residual activity at 20 mM Termitomyces clypeatus
Mg2+ the mycelial extract shows 62.1% residual activity at 20 mM, the purified enzyme shows 39.2% residual activity at 20 mM Termitomyces clypeatus
Mn2+ the mycelial extract shows 42.9% residual activity at 20 mM, the purified enzyme shows 26.3% residual activity at 20 mM Termitomyces clypeatus
p-chloromercuribenzoic acid the mycelial extract shows 66.9% residual activity at 0.5 mM, the purified enzyme shows 36.9% residual activity at 0.5 mM Termitomyces clypeatus
SDS the mycelial extract shows no residual activity at 0.05% (v/v), purified enzyme shows 50% residual activity at 0.05% (v/v) Termitomyces clypeatus
Sodium azide the mycelial extract shows 67.6% residual activity at 20 mM, the purified enzyme shows 42.7% residual activity at 20 mM Termitomyces clypeatus
Triton X-100 the mycelial extract shows 70.6% residual activity at 1% (v/v), the purified enzyme is not inhibited by 1% (v/v) Triton X-100 Termitomyces clypeatus
Urea the mycelial extract shows 8% residual activity at 4 M, the purified enzyme shows 66% residual activity at 4 M Termitomyces clypeatus
Zn2+ the mycelial extract shows 38.6% residual activity at 20 mM, the purified enzyme shows 30.3% residual activity at 20 mM Termitomyces clypeatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.131
-
 4-nitrophenyl-beta-D-glucopyranoside mycelial extract Termitomyces clypeatus
0.148
-
 4-nitrophenyl-beta-D-glucopyranoside purified enzyme Termitomyces clypeatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6688
-
 MALDI-TOF mass spectrometry, in the presence of 2-deoxy-D-glucose Termitomyces clypeatus
116000
-
 SDS-PAGE Termitomyces clypeatus
125900
-
 HPGPLC column chromatography Termitomyces clypeatus

Organism

Organism UniProt Comment Textmining
Termitomyces clypeatus
-
-
-
Termitomyces clypeatus MTCC 5091
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HPGPLC column chromatography, Sephacryl S-200 gel filtration and DEAE Sephadex gel filtration Termitomyces clypeatus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Termitomyces clypeatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
 purified enzyme, using methyl beta-D-glucopyranoside as substrate Termitomyces clypeatus
0.14
-
 purified enzyme, using salicin as substrate Termitomyces clypeatus
0.15
-
 purified enzyme, using cellobiose as substrate Termitomyces clypeatus
0.54
-
 purified enzyme, using 4-nitrophenyl.beta-D-glucopyranoside as substrate Termitomyces clypeatus
43.3
-
 mycelial extract Termitomyces clypeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O the enzyme had higher specific activity against 4-nitrophenyl beta-D-glucopyranoside than cellobiose Termitomyces clypeatus 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O the enzyme had higher specific activity against 4-nitrophenyl beta-D-glucopyranoside than cellobiose Termitomyces clypeatus MTCC 5091 4-nitrophenol + D-glucopyranose
-
 ?
cellobiose + H2O
-
 Termitomyces clypeatus 2 beta-D-glucose
-
 ?
cellobiose + H2O
-
 Termitomyces clypeatus MTCC 5091 2 beta-D-glucose
-
 ?
methyl beta-D-glucopyranoside + H2O
-
 Termitomyces clypeatus ?
-
 ?
methyl beta-D-glucopyranoside + H2O
-
 Termitomyces clypeatus MTCC 5091 ?
-
 ?
additional information the purified enzyme shows substrate specificity only towards substrates with beta-1,4 linkages and displays no hydrolytic activity towards 2-nitrophenyl apha-D-galactopyranoside, methyl alpha-D-galactopyranoside, melibiose, carboxymethyl cellulose, and starch Termitomyces clypeatus ?
-
 ?
additional information the purified enzyme shows substrate specificity only towards substrates with beta-1,4 linkages and displays no hydrolytic activity towards 2-nitrophenyl apha-D-galactopyranoside, methyl alpha-D-galactopyranoside, melibiose, carboxymethyl cellulose, and starch Termitomyces clypeatus MTCC 5091 ?
-
 ?
salicin + H2O
-
 Termitomyces clypeatus ?
-
 ?
salicin + H2O
-
 Termitomyces clypeatus MTCC 5091 ?
-
 ?

Synonyms

Synonyms Comment Organism
cellobiase
-
 Termitomyces clypeatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Termitomyces clypeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 80 the purified enzyme has about 80% and 46% residual activities at 60°C and 80°C, unpurified mycelia extract enzyme has 86%, 60% and 22% residual activities at 40°C, 60°C, and 80°C, respectively. The thermal stability 70°C is 64% Termitomyces clypeatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 residual activity is 80-100% between pH ranges 5.0-8.0, the stability of the enzyme in mycelia extract is comparatively low at acidic pH and the enzyme is most stable at pH 9.0, the purified enzyme shows more than 95% residual activity between pH ranges 6.0-7.0 Termitomyces clypeatus