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Literature summary for 3.2.1.21 extracted from

  • Harnpicharnchai, P.; Champreda, V.; Sornlake, W.; Eurwilaichitr, L.
    A thermotolerant beta-glucosidase isolated from an endophytic fungi, Periconia sp., with a possible use for biomass conversion to sugars (2008), Protein Expr. Purif., 67, 61-69.
    View publication on PubMed

Application

Application Comment Organism
synthesis enzyme is able to hydrolyze rice straw into simple sugars Periconia sp.

Cloned(Commentary)

Cloned (Comment) Organism
-
Periconia sp.

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, 58% residual activity Periconia sp.
Cu2+ 1 mM, 5.1% residual activity Periconia sp.
Fe2+ 1 mM, 30% residual activity Periconia sp.
Zn2+ 1 mM, 51% residual activity Periconia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
4-methylumbelliferyl beta-D-cellobioside pH 5.0, 40°C Periconia sp.
0.19
-
4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 40°C Periconia sp.
0.5
-
cellobiose pH 5.0, 40°C Periconia sp.
4.73
-
4-nitrophenyl-alpha-D-glucopyranoside pH 5.0, 40°C Periconia sp.
11.57
-
4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 40°C Periconia sp.
22.5
-
4-nitrophenyl-beta-D-fucopyranoside pH 5.0, 40°C Periconia sp.

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol at 10%, 107%, at 50%, 0.2% of initial activity, respectively Periconia sp.
Ethanol at 10%, 127%, at 50%, 0.6% of initial activity, respectively Periconia sp.
Methanol at 10%, 121%, at 50%, 1.3% of initial activity, respectively Periconia sp.

Organism

Organism UniProt Comment Textmining
Periconia sp. A9UIG0
-
-

Storage Stability

Storage Stability Organism
4°C, MOPS buffer pH 6.0, stable for at least 6 months Periconia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-cellobioside + 2 H2O
-
Periconia sp. 4-methylumbelliferol + 2 D-glucose
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Periconia sp. 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-arabinopyranoside + H2O
-
Periconia sp. 4-nitrophenol + D-arabinose
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
Periconia sp. 4-nitrophenol + D-fucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Periconia sp. 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Periconia sp. 4-nitrophenol + D-xylose
-
?
4-nitrophenyl-alpha-D-xylopyranoside + H2O
-
Periconia sp. 4-nitrophenol + D-xylose
-
?
cellobiose + H2O
-
Periconia sp. 2 D-glucose
-
?
additional information enzyme is able to hydrolyze rice straw into simple sugars Periconia sp. ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Periconia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermostability is improved in presence of cellobiose, glucose, or sucrose Periconia sp.
70
-
1.5 h, 60% residual activity Periconia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
and 6 Periconia sp.
6
-
and 5 Periconia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
and above, 2 h, no loss of activity Periconia sp.