Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 50% residual activity | Carapichea ipecacuanha | |
Fe3+ | 1 mM, 46% residual activity | Carapichea ipecacuanha | |
additional information | not inhibitory: EDTA, 2-mercaptoethanol | Carapichea ipecacuanha | |
Zn2+ | 1 mM, 64% residual activity | Carapichea ipecacuanha |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ipecoside | pH 5.0, 30°C | Carapichea ipecacuanha | |
0.19 | - |
strictosidine | pH 5.0, 30°C | Carapichea ipecacuanha | |
0.29 | - |
vincoside | pH 5.0, 30°C | Carapichea ipecacuanha | |
0.32 | - |
N-deacetylipecoside | pH 5.0, 30°C | Carapichea ipecacuanha | |
0.39 | - |
6-O-methyl-N-deacetylipecoside | pH 5.0, 30°C | Carapichea ipecacuanha |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carapichea ipecacuanha | B6ZKM3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-O-methyl-N-deacetylipecoside + H2O | 90.5% of the efficiency with N-deacetylipecoside | Carapichea ipecacuanha | ? | - |
? | |
ipecoside + H2O | 586% of the efficiency with N-deacetylipecoside | Carapichea ipecacuanha | ? | - |
? | |
additional information | isoform IpeGlu1 preferentially hydrolyzes glucosidic Ipecac alkaloids except for their lactams, but shows poor or no activity toward other substrates, including terpenoid-indole alkaloid glucosides. IpeGlu1 activity is extremely poor toward 7-O-methyl and 6,7-O,O-dimethyl derivatives. 6-O-methyl derivatives are hydrolyzed as efficiently as non-methylated substrates. IpeGlu1 lacks stereospecificity for its substrates. 1(R)-epimers are preferred to 1(S)-epimers | Carapichea ipecacuanha | ? | - |
? | |
N-deacetylipecoside + H2O | - |
Carapichea ipecacuanha | ? | - |
? | |
strictosidine + H2O | 12.6% of the efficiency with N-deacetylipecoside | Carapichea ipecacuanha | ? | - |
? | |
vincoside + H2O | 25.1% of the efficiency with N-deacetylipecoside | Carapichea ipecacuanha | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glu1 | - |
Carapichea ipecacuanha |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
strictosidine | pH 5.0, 30°C | Carapichea ipecacuanha | |
6.4 | - |
vincoside | pH 5.0, 30°C | Carapichea ipecacuanha | |
28.2 | - |
N-deacetylipecoside | pH 5.0, 30°C | Carapichea ipecacuanha | |
31.1 | - |
6-O-methyl-N-deacetylipecoside | pH 5.0, 30°C | Carapichea ipecacuanha | |
51.6 | - |
ipecoside | pH 5.0, 30°C | Carapichea ipecacuanha |