Cloned (Comment) | Organism |
---|---|
expression of N-terminally His6-tagged cytosolic isozyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinnat enzyme, hanging drop vapour diffusion method, micro-seeding, the protein crystallized in 1.6 M ammonium sulfate, 100 mM HEPES, pH 7.0, within 4 weeks, X-ray diffraction structure determination and analysis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
4-methylumbelliferyl-beta-D-glucopyranoside | pH 7.0, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46500 | - |
recombinant His-tagged enzyme, gel filtration | Homo sapiens |
55000 | - |
1 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 57346, mass spectrometry | Homo sapiens |
57346 | - |
1 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 57346, mass spectrometry | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged cytosolic isozyme from Sf9 insect cells by nickel affinity and ion exchange chromatography to homogeneity | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.97 | - |
purified recombinant His-tagged enzyme, pH 5.7 | Homo sapiens |
2.62 | - |
purified recombinant His-tagged enzyme, pH 7.0 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 57346, mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CBG | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | 7 | assay at | Homo sapiens |