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Literature summary for 3.2.1.21 extracted from
- Characterization and comparison of thermostability of purified beta-glucosidases from a mesophilic Aureobasidium pullulans and a thermophilic Thermoascus aurantiacus (2007), Process Biochem., 42, 1101-1106.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics and thermodynamics | Thermoascus aurantiacus | |
| additional information | - |
additional information | kinetics and thermodynamics | Aureobasidium pullulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Thermoascus aurantiacus | an important enzyme of the cellulolytic complex necessary for the complete degrading of cellulose | ? | - |
? |  |
| additional information | Aureobasidium pullulans | an important enzyme of the cellulolytic complex necessary for the complete degrading of cellulose | ? | - |
? | |
| additional information | Aureobasidium pullulans ER-16 | an important enzyme of the cellulolytic complex necessary for the complete degrading of cellulose | ? | - |
? | |
| additional information | Thermoascus aurantiacus 179-5 | an important enzyme of the cellulolytic complex necessary for the complete degrading of cellulose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aureobasidium pullulans | - |
- |
- |
| Aureobasidium pullulans ER-16 | - |
- |
- |
| Thermoascus aurantiacus | - |
- |
- |
| Thermoascus aurantiacus 179-5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 35.5fold to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange chromatography, gel filtration, and ultrafiltration | Thermoascus aurantiacus |
| native enzyme 46.3fold to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange chromatography, gel filtration, and ultrafiltration | Aureobasidium pullulans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | cultivation in corn-cob by solid state fermentation | Thermoascus aurantiacus | - |
| additional information | cultivation in corn-cob by solid state fermentation | Aureobasidium pullulans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | an important enzyme of the cellulolytic complex necessary for the complete degrading of cellulose | Thermoascus aurantiacus | ? | - |
? | |
| additional information | an important enzyme of the cellulolytic complex necessary for the complete degrading of cellulose | Aureobasidium pullulans | ? | - |
? | |
| additional information | an important enzyme of the cellulolytic complex necessary for the complete degrading of cellulose | Aureobasidium pullulans ER-16 | ? | - |
? | |
| additional information | an important enzyme of the cellulolytic complex necessary for the complete degrading of cellulose | Thermoascus aurantiacus 179-5 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiase | - |
Thermoascus aurantiacus |
| cellobiase | - |
Aureobasidium pullulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermoascus aurantiacus |
| 80 | - |
- |
Aureobasidium pullulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermoinactivation followed first-order kinetics, overview | Thermoascus aurantiacus |
| additional information | - |
thermoinactivation followed first-order kinetics, overview | Aureobasidium pullulans |
| 70 | - |
1 h, purified enzyme, 98% remaining activity | Thermoascus aurantiacus |
| 75 | - |
1 h, purified enzyme, 98% remaining activity | Aureobasidium pullulans |
| 80 | - |
purified enzyme, half-life is 30 min | Thermoascus aurantiacus |
| 80 | - |
purified enzyme, half-life is 90 min | Aureobasidium pullulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | - |
Aureobasidium pullulans |
| 4.5 | - |
- |
Thermoascus aurantiacus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | purified enzyme, stable | Aureobasidium pullulans |
| 4.5 | 6.5 | purified enzyme, stable | Thermoascus aurantiacus |
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