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Literature summary for 3.2.1.21 extracted from

  • Hong, S.Y.; Cho, K.M.; Math, R.K.; Kim, Y.H.; Hong, S.J.; Cho, Y.U.; Kim, H.; Yun, H.D.
    Characterization of the recombinant cellobiase from celG gene in the beta-glucoside utilization gene operon of Pectobacterium carotovorum subsp. carotovorum LY34 (2007), J. Mol. Catal. B, 47, 91-98.
No PubMed abstract available
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
gene celG encoded in the celEFG operon, construction of a cosmid library and cloning of the celEFG operon, genetic organizatin, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Pectobacterium carotovorum

Protein Variants

Protein Variants Comment Organism
E172A site-directed mutagenesis, inactive catalytic residue mutant Pectobacterium carotovorum
E370A site-directed mutagenesis, inactive catalytic residue mutant Pectobacterium carotovorum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Pectobacterium carotovorum
Co2+
-
 Pectobacterium carotovorum
Cu2+
-
 Pectobacterium carotovorum
Hg2+
-
 Pectobacterium carotovorum
Mg2+
-
 Pectobacterium carotovorum
Mn2+
-
 Pectobacterium carotovorum
Zn2+
-
 Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, CelG, SDS-PAGE Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Pectobacterium carotovorum
-
 2 beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum A1C3J9 CelG, beta-glucosidase; subsp. carotovorum, strain LY34, gene celG
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and two steps of anion exchange chromatography Pectobacterium carotovorum

Reaction

Reaction Comment Organism Reaction ID
celloheptaose + 6 H2O = 7 beta-D-glucose the two glutamic acid residues Glu172 and Glu370 are essential for enzyme activity of CellG Pectobacterium carotovorum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Pectobacterium carotovorum 4-methylumbelliferol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pectobacterium carotovorum 4-nitrophenol + beta-D-glucose
-
 ?
arbutin + H2O
-
 Pectobacterium carotovorum ?
-
 ?
cellobiose + H2O
-
 Pectobacterium carotovorum 2 beta-D-glucose
-
 ?
salicin + H2O
-
 Pectobacterium carotovorum beta-D-glucose + 2-(hydroxymethyl)phenol
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, CelG, SDS-PAGE Pectobacterium carotovorum

Synonyms

Synonyms Comment Organism
cellobiase
-
 Pectobacterium carotovorum
More the enzyme belongs to the glycosyl hydrolase family 1 Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pectobacterium carotovorum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80
-
 Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pectobacterium carotovorum

pH Range

pH Minimum pH Maximum Comment Organism
4 9 activity range of CelG Pectobacterium carotovorum