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Literature summary for 3.2.1.21 extracted from
- Enzymatic properties of two beta-glucosidases from Ceriporiopsis subvermispora produced in biopulping conditions (2006), J. Appl. Microbiol., 101, 480-486.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Gelatoporia subvermispora |  |
| Cu2+ | - |
Gelatoporia subvermispora | |
| D-glucose | - |
Gelatoporia subvermispora | |
| EDTA | slight inhibition | Gelatoporia subvermispora | |
| KCl | - |
Gelatoporia subvermispora | |
| MgCl2 | - |
Gelatoporia subvermispora | |
| SDS | - |
Gelatoporia subvermispora | |
| Urea | - |
Gelatoporia subvermispora | |
| ZnSO4 | - |
Gelatoporia subvermispora | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.63 | - |
4-nitrophenyl-beta-D-glucopyranoside | large isozyme, pH 3.5, 60°C | Gelatoporia subvermispora | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | activates | Gelatoporia subvermispora | |
| Mn2+ | activates | Gelatoporia subvermispora | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
small isozyme, gel filtration | Gelatoporia subvermispora |
| 110000 | - |
large isozyme, gel filtration | Gelatoporia subvermispora |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | Gelatoporia subvermispora | - |
2 beta-D-glucose | - |
? | |
| cellobiose + H2O | Gelatoporia subvermispora CS-1 | - |
2 beta-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gelatoporia subvermispora | - |
- |
- |
| Gelatoporia subvermispora CS-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native large isozyme 3.7fold by successive gel filtration | Gelatoporia subvermispora |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | cultivation on cellulose or Pinus taeda wood, biopulping conditions, optimization and evaluation, overview | Gelatoporia subvermispora | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gelatoporia subvermispora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Gelatoporia subvermispora | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Gelatoporia subvermispora CS-1 | 4-nitrophenol + beta-D-glucose | - |
? | |
| cellobiose + H2O | - |
Gelatoporia subvermispora | 2 beta-D-glucose | - |
? | |
| cellobiose + H2O | - |
Gelatoporia subvermispora CS-1 | 2 beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
large isozyme | Gelatoporia subvermispora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
large isozyme | Gelatoporia subvermispora |
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Release 2023.1 (January 2023)
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