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Literature summary for 3.2.1.21 extracted from
- Purification and biochemical characterization of an extracellular beta-glucosidase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm (2007), FEMS Microbiol. Lett., 270, 162-170.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | slight activation | Daldinia eschscholtzii |  |
| additional information | poor activation by glycerol, dimethyl sulfoxide, and dithiothreitol | Daldinia eschscholtzii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | competitive inhibition | Daldinia eschscholtzii | |
| Hg2+ | 93% inhibition at 1 mM | Daldinia eschscholtzii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | 2 | 4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Daldinia eschscholtzii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Daldinia eschscholtzii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates slightly | Daldinia eschscholtzii | |
| Mg2+ | activates 22% at 1 mM | Daldinia eschscholtzii | |
| Mn2+ | activates 18% at 1 mM | Daldinia eschscholtzii | |
| additional information | not affected by Co2+ | Daldinia eschscholtzii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64200 | - |
1 * 64200, SDS-PAGE | Daldinia eschscholtzii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daldinia eschscholtzii | - |
a wood-decaying fungus, collected from a dead mango tree | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 50fold from culture filtrate by ammonium sulfate precipitation, cation-exchange and hydrophobic interaction chromatography, and gel filtration | Daldinia eschscholtzii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | growth on 1.0% w/v carboxymethyl-cellulose | Daldinia eschscholtzii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 77.86 | - |
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside | Daldinia eschscholtzii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Daldinia eschscholtzii | 4-nitrophenol + beta-D-glucose | - |
? | |
| cellobiose + H2O | - |
Daldinia eschscholtzii | 2 beta-D-glucose | - |
? | |
| gentiobiose + H2O | - |
Daldinia eschscholtzii | 2 beta-D-glucose | - |
? | |
| laminaribiose + H2O | - |
Daldinia eschscholtzii | 2 beta-D-glucose | - |
? | |
| additional information | substrate specificity, overview, the enzyme catalyzes the hydrolysis of beta-glucosidic linkages, such as those in alkyl- or aryl-beta-glucosides as well as diglucosides and oligosaccharides, no activity with lactose, sucrose, Avicel, or 2-nitrophenyl-beta-D-galactopyranoside | Daldinia eschscholtzii | ? | - |
? | |
| sophorose + H2O | - |
Daldinia eschscholtzii | 2 beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64200, SDS-PAGE | Daldinia eschscholtzii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Daldinia eschscholtzii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | temperature profile | Daldinia eschscholtzii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, stable up to, pH 5.0 | Daldinia eschscholtzii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Daldinia eschscholtzii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH profile | Daldinia eschscholtzii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.79 | - |
D-glucose | pH 5.0, 50°C | Daldinia eschscholtzii | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Daldinia eschscholtzii | isoelectric focusing | - |
8.55 |
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