Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Melanocarpus sp. | |
DTT | - |
Melanocarpus sp. | |
ethanol | - |
Melanocarpus sp. | |
methanol | - |
Melanocarpus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Melanocarpus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C | Melanocarpus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Melanocarpus sp. | |
K+ | activates | Melanocarpus sp. | |
Mg2+ | activates | Melanocarpus sp. | |
Na+ | activates | Melanocarpus sp. | |
Zn2+ | activates | Melanocarpus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
1 * 92000, SDS-PAGE | Melanocarpus sp. |
102000 | - |
gel filtration | Melanocarpus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Melanocarpus sp. | - |
thermophilic fungus | - |
Melanocarpus sp. MTCC 3922 | - |
thermophilic fungus | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity | Melanocarpus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + 2 H2O | low activity | Melanocarpus sp. | 4-nitrophenol + 2 beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-cellobioside + 2 H2O | low activity | Melanocarpus sp. MTCC 3922 | 4-nitrophenol + 2 beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Melanocarpus sp. | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Melanocarpus sp. MTCC 3922 | 4-nitrophenol + beta-D-glucose | - |
? | |
additional information | the enzyme exhibits transglycosylation activity with methanol as acceptor | Melanocarpus sp. | ? | - |
? | |
additional information | the enzyme exhibits transglycosylation activity with methanol as acceptor | Melanocarpus sp. MTCC 3922 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 92000, SDS-PAGE | Melanocarpus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Melanocarpus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | - |
Melanocarpus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, stable | Melanocarpus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
66.67 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.0, 60°C | Melanocarpus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Melanocarpus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | purified enzyme, stable | Melanocarpus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Melanocarpus sp. | - |
- |
4.1 |