Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strain PN-120 produces a beta-glucosidase that is 10fold more active with a 3fold higher affinity for cellobiose than the corresponding enzyme isolated from the parental strain, the mutation of strain PN-120 affects the catalytic site | Cellulomonas flavigena |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.25 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.2, 40°C, mutant enzyme | Cellulomonas flavigena | |
7.1 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.2, 40°C, wild-type enzyme | Cellulomonas flavigena | |
37 | - |
cellobiose | pH 6.2, 40°C, mutant enzyme | Cellulomonas flavigena | |
114 | - |
cellobiose | pH 6.2, 40°C, wild-type enzyme | Cellulomonas flavigena |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
gel filtration | Cellulomonas flavigena |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Cellulomonas flavigena | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas flavigena | - |
wild-type strain CDBB531 and mutant strain PN-120 | - |
Purification (Comment) | Organism |
---|---|
native wild-type and mutant enzymes partially by anion exchange chromatography and gel filtration 29fold and 16fold, respectively | Cellulomonas flavigena |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | mutant strain PN-120 produces a beta-glucosidase that is 16fold more active than the corresponding enzyme isolated from the parental strain | Cellulomonas flavigena | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.27 | - |
partially purified wild-type enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside | Cellulomonas flavigena |
14.1 | - |
partially purified mutant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside | Cellulomonas flavigena |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Cellulomonas flavigena | 4-methylumbelliferol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Cellulomonas flavigena | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Cellulomonas flavigena | 2 beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | three protein bands in SDS-PAGE | Cellulomonas flavigena |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | wild-type and mutant enzymes | Cellulomonas flavigena |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | - |
Cellulomonas flavigena |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
wild-type and mutant enzymes | Cellulomonas flavigena |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Cellulomonas flavigena |