Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type Bgl3B in Escherichia coli strain Tuner (DE3), and of His-tagged selenomethionine-labeled enzyme in Escherichia coli strain B834 (DE3) | Thermotoga neapolitana |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-substituted enzymes, hanging drop vapour diffusion method, 3-5 mg/ml protein in 20 mM MES buffer, pH 6.2, crystallization in microplates at 20°C within 4 days, precipitant is 90 mM bis-Tris propane buffer, pH 7.4, with 16-20% PEG 3350, 0.1-0.2 M NaI for the wild-type enzyme, and 20-24% PEG 3350, 0.1-0.25 M NaI for the selenomethionine-labeled enzyme, mixture of 0.001 ml of protein and reservoir solution, X-ray diffraction structure determination and analysis at 2.4 A resolution, MAD method, model building | Thermotoga neapolitana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermotoga neapolitana | the enzyme plays a role in the carbohydrate metabolism of many organisms by acting on the beta-glycosidic linkages of cellooligosaccharides containing beta-D-1,4-glycosidic bonds, glycosyl hydrolase family 3 enzymes are retaining enzymes that utilize a double-displacement mechanism with retention of configuration at the anomeric carbon of the sugar ring and often display transglycosylation abilities | ? | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | - |
DSM strain 4359 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and selenomethionine-labeld enzymes from Escherichia coli by immobilized metal-ion affinity chromatography column | Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme plays a role in the carbohydrate metabolism of many organisms by acting on the beta-glycosidic linkages of cellooligosaccharides containing beta-D-1,4-glycosidic bonds, glycosyl hydrolase family 3 enzymes are retaining enzymes that utilize a double-displacement mechanism with retention of configuration at the anomeric carbon of the sugar ring and often display transglycosylation abilities | Thermotoga neapolitana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase B | - |
Thermotoga neapolitana |
Bgl3B | - |
Thermotoga neapolitana |
More | the enzyme belongs to the glycosyl hydrolase family 3 | Thermotoga neapolitana |