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Literature summary for 3.2.1.21 extracted from

  • Turner, P.; Pramhed, A.; Kanders, E.; Hedstroem, M.; Karlsson, E.N.; Logan, D.T.
    Expression, purification, crystallization and preliminary X-ray diffraction analysis of Thermotoga neapolitana beta-glucosidase B (2007), Acta Crystallogr. Sect. F, 63, 802-806.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type Bgl3B in Escherichia coli strain Tuner (DE3), and of His-tagged selenomethionine-labeled enzyme in Escherichia coli strain B834 (DE3) Thermotoga neapolitana

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and selenomethionine-substituted enzymes, hanging drop vapour diffusion method, 3-5 mg/ml protein in 20 mM MES buffer, pH 6.2, crystallization in microplates at 20°C within 4 days, precipitant is 90 mM bis-Tris propane buffer, pH 7.4, with 16-20% PEG 3350, 0.1-0.2 M NaI for the wild-type enzyme, and 20-24% PEG 3350, 0.1-0.25 M NaI for the selenomethionine-labeled enzyme, mixture of 0.001 ml of protein and reservoir solution, X-ray diffraction structure determination and analysis at 2.4 A resolution, MAD method, model building Thermotoga neapolitana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermotoga neapolitana the enzyme plays a role in the carbohydrate metabolism of many organisms by acting on the beta-glycosidic linkages of cellooligosaccharides containing beta-D-1,4-glycosidic bonds, glycosyl hydrolase family 3 enzymes are retaining enzymes that utilize a double-displacement mechanism with retention of configuration at the anomeric carbon of the sugar ring and often display transglycosylation abilities ?
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?

Organism

Organism UniProt Comment Textmining
Thermotoga neapolitana
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DSM strain 4359
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and selenomethionine-labeld enzymes from Escherichia coli by immobilized metal-ion affinity chromatography column Thermotoga neapolitana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme plays a role in the carbohydrate metabolism of many organisms by acting on the beta-glycosidic linkages of cellooligosaccharides containing beta-D-1,4-glycosidic bonds, glycosyl hydrolase family 3 enzymes are retaining enzymes that utilize a double-displacement mechanism with retention of configuration at the anomeric carbon of the sugar ring and often display transglycosylation abilities Thermotoga neapolitana ?
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?

Synonyms

Synonyms Comment Organism
beta-glucosidase B
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Thermotoga neapolitana
Bgl3B
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Thermotoga neapolitana
More the enzyme belongs to the glycosyl hydrolase family 3 Thermotoga neapolitana