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Literature summary for 3.2.1.21 extracted from

  • Nakkharat, P.; Haltrich, D.
    Purification and characterisation of an intracellular enzyme with beta-glucosidase and beta-galactosidase activity from the thermophilic fungus Talaromyces thermophilus CBS 236.58 (2006), J. Biotechnol., 123, 304-313.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cellobiose substrate inhibition at 10 mM Thermomyces dupontii
Cu2+
-
Thermomyces dupontii
D-fructose
-
Thermomyces dupontii
D-galactose competitive product inhibition Thermomyces dupontii
D-glucose competitive product inhibition Thermomyces dupontii
D-xylose
-
Thermomyces dupontii
L-arabinose
-
Thermomyces dupontii
lactose
-
Thermomyces dupontii
maltose
-
Thermomyces dupontii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics Thermomyces dupontii
1.2
-
2-Nitrophenyl-beta-D-glucopyranoside pH 6.5, 40°C Thermomyces dupontii
5.7
-
cellobiose pH 6.5, 40°C Thermomyces dupontii
24
-
2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 40°C Thermomyces dupontii

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Thermomyces dupontii 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Thermomyces dupontii
Co2+ activates Thermomyces dupontii
Fe2+ activates Thermomyces dupontii
K+ activates Thermomyces dupontii
Mg2+ activates Thermomyces dupontii
Mn2+ activates Thermomyces dupontii
Na+ activates Thermomyces dupontii
Zn2+ activates Thermomyces dupontii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
gel filtration Thermomyces dupontii
50000
-
1 * 50000, SDS-PAGE Thermomyces dupontii

Organism

Organism UniProt Comment Textmining
Thermomyces dupontii
-
-
-
Thermomyces dupontii CBS 236.58
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 10.2fold to homogeneity by anion exchange chromatography, ultrafiltration, and affinity chromatography using 4-aminobenzyl, 1-thio-beta-D-galactopyranoside Thermomyces dupontii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
139
-
purified enzyme Thermomyces dupontii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermomyces dupontii 2-nitrophenol + beta-D-galactose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermomyces dupontii CBS 236.58 2-nitrophenol + beta-D-galactose
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermomyces dupontii 2-nitrophenol + beta-D-glucose
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermomyces dupontii CBS 236.58 2-nitrophenol + beta-D-glucose
-
?
cellobiose + H2O
-
Thermomyces dupontii 2 beta-D-glucose
-
?
additional information the enzyme shows significant transglucosylation activity, resulting in formation of e.g. galactooligosaccharides with lactose as acceptor, and is active with galactosides, overview Thermomyces dupontii ?
-
?
additional information the enzyme shows significant transglucosylation activity, resulting in formation of e.g. galactooligosaccharides with lactose as acceptor, and is active with galactosides, overview Thermomyces dupontii CBS 236.58 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Thermomyces dupontii

Synonyms

Synonyms Comment Organism
BGL1
-
Thermomyces dupontii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Thermomyces dupontii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
167
-
cellobiose pH 6.5, 40°C Thermomyces dupontii
304
-
2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 40°C Thermomyces dupontii
308
-
2-Nitrophenyl-beta-D-glucopyranoside pH 6.5, 40°C Thermomyces dupontii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Thermomyces dupontii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
66
-
D-glucose versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C Thermomyces dupontii
370
-
D-galactose versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C Thermomyces dupontii

pI Value

Organism Comment pI Value Maximum pI Value
Thermomyces dupontii isoelectric focusing 4.6 4.5