Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | substrate inhibition at 10 mM | Thermomyces dupontii | |
Cu2+ | - |
Thermomyces dupontii | |
D-fructose | - |
Thermomyces dupontii | |
D-galactose | competitive product inhibition | Thermomyces dupontii | |
D-glucose | competitive product inhibition | Thermomyces dupontii | |
D-xylose | - |
Thermomyces dupontii | |
L-arabinose | - |
Thermomyces dupontii | |
lactose | - |
Thermomyces dupontii | |
maltose | - |
Thermomyces dupontii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Thermomyces dupontii | |
1.2 | - |
2-Nitrophenyl-beta-D-glucopyranoside | pH 6.5, 40°C | Thermomyces dupontii | |
5.7 | - |
cellobiose | pH 6.5, 40°C | Thermomyces dupontii | |
24 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 40°C | Thermomyces dupontii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Thermomyces dupontii | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Thermomyces dupontii | |
Co2+ | activates | Thermomyces dupontii | |
Fe2+ | activates | Thermomyces dupontii | |
K+ | activates | Thermomyces dupontii | |
Mg2+ | activates | Thermomyces dupontii | |
Mn2+ | activates | Thermomyces dupontii | |
Na+ | activates | Thermomyces dupontii | |
Zn2+ | activates | Thermomyces dupontii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
gel filtration | Thermomyces dupontii |
50000 | - |
1 * 50000, SDS-PAGE | Thermomyces dupontii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces dupontii | - |
- |
- |
Thermomyces dupontii CBS 236.58 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 10.2fold to homogeneity by anion exchange chromatography, ultrafiltration, and affinity chromatography using 4-aminobenzyl, 1-thio-beta-D-galactopyranoside | Thermomyces dupontii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
139 | - |
purified enzyme | Thermomyces dupontii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermomyces dupontii | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermomyces dupontii CBS 236.58 | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermomyces dupontii | 2-nitrophenol + beta-D-glucose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermomyces dupontii CBS 236.58 | 2-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Thermomyces dupontii | 2 beta-D-glucose | - |
? | |
additional information | the enzyme shows significant transglucosylation activity, resulting in formation of e.g. galactooligosaccharides with lactose as acceptor, and is active with galactosides, overview | Thermomyces dupontii | ? | - |
? | |
additional information | the enzyme shows significant transglucosylation activity, resulting in formation of e.g. galactooligosaccharides with lactose as acceptor, and is active with galactosides, overview | Thermomyces dupontii CBS 236.58 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Thermomyces dupontii |
Synonyms | Comment | Organism |
---|---|---|
BGL1 | - |
Thermomyces dupontii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Thermomyces dupontii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
167 | - |
cellobiose | pH 6.5, 40°C | Thermomyces dupontii | |
304 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 40°C | Thermomyces dupontii | |
308 | - |
2-Nitrophenyl-beta-D-glucopyranoside | pH 6.5, 40°C | Thermomyces dupontii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Thermomyces dupontii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
66 | - |
D-glucose | versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C | Thermomyces dupontii | |
370 | - |
D-galactose | versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C | Thermomyces dupontii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomyces dupontii | isoelectric focusing | 4.6 | 4.5 |