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Literature summary for 3.2.1.21 extracted from

  • Michelin, K.; Wajner, A.; Goulart, L.d.S.; Fachel, A.A.; Pereira, M.L.; de Mello, A.S.; Souza, F.T.; Pires, R.F.; Giugliani, R.; Coelho, J.C.
    Biochemical study on beta-glucosidase in individuals with Gaucher's disease and normal subjects (2004), Clin. Chim. Acta, 343, 145-153.
    View publication on PubMed
Search for more literature for 3.2.1.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens
3.64
-
 4-methylumbelliferyl beta-D-glucoside pH 5.4, 37°C, Gaucher's disease enzyme from leukocytes Homo sapiens
4.13
-
 4-methylumbelliferyl beta-D-glucoside pH 5.4, 37°C, healthy human enzyme from leukocytes Homo sapiens
6.18
-
 4-methylumbelliferyl beta-D-glucoside pH 5.4, 37°C, healthy human enzyme from fibroblasts Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme deficiency causes the Gaucher's disease, the enzyme participates in degradation of glycosphingolipids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 healthy humans and Gaucher's disease patients
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast from healthy humans and Gaucher's disease patients Homo sapiens
-
leukocyte from healthy humans and Gaucher's disease patients Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-glucoside + H2O
-
 Homo sapiens 4-methylumbelliferol + beta-D-glucose
-
 ?
additional information enzyme deficiency causes the Gaucher's disease, the enzyme participates in degradation of glycosphingolipids Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life of the healthy leukocyte enzyme is 3.5 min, of the healthy fibroblast 3 min, and half-life of the Gaucher's disease enzyme from leukocyte is about 6 min, and from fibroblast 3 min, thermostability, overview Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.9 5.3 healthy human enzyme Homo sapiens
5.2 5.4 Gaucher's disease enzyme Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
10.3
-
 inactivation Homo sapiens