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Literature summary for 3.2.1.21 extracted from

  • Li, X.; Pei, J.; Wu, G.; Shao, W.
    Expression, purification and characterization of a recombinant beta-glucosidase from Volvariella volvacea (2005), Biotechnol. Lett., 27, 1369-1373.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is unaffected by EDTA and Tween 20 Volvariella volvacea
Triton X-100
-
 Volvariella volvacea

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Volvariella volvacea

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition Volvariella volvacea
Hg2+ complete inhibition Volvariella volvacea
additional information the enzyme is unaffected by EDTA and Tween 20 Volvariella volvacea
Ni2+
-
 Volvariella volvacea
SDS complete inhibition Volvariella volvacea
Zn2+
-
 Volvariella volvacea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 4-nitrophenyl beta-D-glucoside pH 6.4, 50°C, recombinant enzyme Volvariella volvacea

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is unaffected by Mn2+, Mg2+, and Co2+ Volvariella volvacea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 4 * 97000, recombinant enzyme, SDS-PAGE Volvariella volvacea
380000
-
 recombinant enzyme, gel filtration Volvariella volvacea

Organism

Organism UniProt Comment Textmining
Volvariella volvacea Q9C3Z9
-
-
Volvariella volvacea V1-1 Q9C3Z9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 16fold to homogeneity by ion exchange chromatography and gel filtration Volvariella volvacea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
96
-
 purified recombinant enzyme Volvariella volvacea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Volvariella volvacea 4-nitrophenol + D-xylose
-
 ?
4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Volvariella volvacea V1-1 4-nitrophenol + D-xylose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Volvariella volvacea ?
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Volvariella volvacea V1-1 ?
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Volvariella volvacea 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Volvariella volvacea V1-1 4-nitrophenol + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Volvariella volvacea beta-D-glucose + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Volvariella volvacea V1-1 beta-D-glucose + beta-D-glucose
-
 ?
additional information no activity with oat spelt xylan, 4-nitrophenyl-alpha-D-arabinofuranoside, and 4-nitrophenyl-beta-D-xylanopyranoside Volvariella volvacea ?
-
 ?
additional information no activity with oat spelt xylan, 4-nitrophenyl-alpha-D-arabinofuranoside, and 4-nitrophenyl-beta-D-xylanopyranoside Volvariella volvacea V1-1 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 97000, recombinant enzyme, SDS-PAGE Volvariella volvacea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Volvariella volvacea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 purified recombinant enzyme, half-life 1 h Volvariella volvacea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Volvariella volvacea

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.6 8 stable, purified recombinant enzyme Volvariella volvacea