Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-nitrophenyl-beta-D-fucopyranoside | competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | |
4-nitrophenyl-beta-D-fucopyranoside | competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | |
D-glucose | competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | |
gluconolactone | competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.096 | - |
4-methylumbelliferyl beta-D-glucoside | pH 7.3, 40°C | Komagataella pastoris | |
0.12 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 7.3, 40°C | Komagataella pastoris | |
0.22 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 7.3, 40°C | Komagataella pastoris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Komagataella pastoris | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
4 * 70000, SDS-PAGE | Komagataella pastoris |
275000 | - |
gel filtration | Komagataella pastoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Komagataella pastoris X-33 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by ammonium sulfate fractionation, gel filtration, ion exchange chromatography, and adsorption chromatography | Komagataella pastoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
purified enzyme | Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | 131% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | 2-nitrophenol + beta-D-glucose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | 131% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris X-33 | 2-nitrophenol + beta-D-glucose | - |
? | |
4-methylumbelliferyl beta-D-glucoside + H2O | 63% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | 4-methylumbelliferol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Komagataella pastoris | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl-1-thio-beta-D-glucopyranoside + H2O | 31% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | 4-nitrophenol + 1-thio-beta-D-glucose | - |
? | |
amygdalin + H2O | 11.3% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | ? | - |
? | |
amygdalin + H2O | 11.3% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris X-33 | ? | - |
? | |
beta-gentiobiose + H2O | 6.2% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | 2 D-glucose | - |
? | |
D-(+)-cellobiose + H2O | 12.9% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | beta-D-glucose | - |
? | |
linamarin + H2O | 34% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | alpha-hydroxyisobutyronitrile + D-glucose | - |
? | |
additional information | no activity with arbutin | Komagataella pastoris | ? | - |
? | |
additional information | no activity with arbutin | Komagataella pastoris X-33 | ? | - |
? | |
n-dodecyl-beta-D-glucopyranoside + H2O | 26% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | n-dodecanol + D-Glucose | - |
? | |
n-octyl-beta-D-glucopyranoside + H2O | 28% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | n-octanol + D-glucose | - |
? | |
n-octyl-beta-D-glucopyranoside + H2O | 28% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris X-33 | n-octanol + D-glucose | - |
? | |
prunasin + H2O | 27% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | D-mandelonitrile + beta-D-glucose | - |
? | |
prunasin + H2O | 27% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris X-33 | D-mandelonitrile + beta-D-glucose | - |
? | |
salicin + H2O | 10.4% of the activity with 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | saligenin + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 70000, SDS-PAGE | Komagataella pastoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Komagataella pastoris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.2 | - |
4-methylumbelliferyl beta-D-glucoside | pH 7.3, 40°C | Komagataella pastoris | |
11.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 7.3, 40°C | Komagataella pastoris | |
13.7 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 7.3, 40°C | Komagataella pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Komagataella pastoris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | fairly stable | Komagataella pastoris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
D-glucose | pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris | |
0.41 | - |
2-nitrophenyl-beta-D-fucopyranoside | pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside | Komagataella pastoris |