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Literature summary for 3.2.1.21 extracted from
- Substrate specificity and transglycosylation catalyzed by a thermostable beta-glucosidase from marine hyperthermophile Thermotoga neapolitana (2005), Appl. Microbiol. Biotechnol., 69, 411-422.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Thermotoga neapolitana |  |
| DTT | - |
Thermotoga neapolitana | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bglA, expression of the His-tagged enzyme in Escherichia coli | Thermotoga neapolitana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Thermotoga neapolitana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not affected by metal ions | Thermotoga neapolitana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56200 | - |
mass spectrometry | Thermotoga neapolitana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | Thermotoga neapolitana | - |
2 D-glucose | - |
? | |
| lactose + H2O | Thermotoga neapolitana | - |
D-glucose + D-galactose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | - |
gene bglA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by heat treatment at 75°C, and nickel affinity chromatography | Thermotoga neapolitana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga neapolitana | 4-nitrophenol + beta-D-glucose | - |
? | |
| cellobiose + H2O | - |
Thermotoga neapolitana | 2 D-glucose | - |
? | |
| cellobiose + H2O | recombinant enzyme | Thermotoga neapolitana | 2 D-glucose | - |
? | |
| gentiobiose + H2O | recombinant enzyme | Thermotoga neapolitana | D-glucose | - |
? | |
| lactose + H2O | - |
Thermotoga neapolitana | D-glucose + D-galactose | - |
? | |
| lactose + H2O | recombinant enzyme | Thermotoga neapolitana | D-glucose + D-galactose | - |
? | |
| laminaribiose + H2O | recombinant enzyme | Thermotoga neapolitana | D-glucose | - |
? | |
| additional information | the enzyme also shows glycosyl tranferase activity with arbutin and cellobiose as acceptor and donor, respectively, resulting in three distinct transfer products, where glucose is transferred from cellobiose to C3, C4, and C5 of arbutin, product analysis by mass spectrometry | Thermotoga neapolitana | ? | - |
? | |
| sophorose + H2O | recombinant enzyme | Thermotoga neapolitana | 2 D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BglA | - |
Thermotoga neapolitana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
half-life is 3.6 h, recombinant enzyme | Thermotoga neapolitana |
| 105 | - |
half-life is 12 min, recombinant enzyme | Thermotoga neapolitana |
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