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Literature summary for 3.2.1.21 extracted from
- Purification and partial characterization of beta-glucosidase from fresh leaves of tea plants (Camellia sinensis (L.) O. Kuntze) (2005), Acta Biochim. Biophys. Sin., 37, 363-370.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Camellia sinensis |
| 41000 | - |
1 * 41000, SDS-PAGE | Camellia sinensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camellia sinensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 117fold to homogeneity from fresh leaves by chilled acetone and ammonium sulfate precipitation, ion exchange chromatography and gel filtration | Camellia sinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Camellia sinensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.57 | - |
purified enzyme | Camellia sinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-galactopyranoside + H2O | low activity | Camellia sinensis | 4-nitrophenol + beta-D-galactose | - |
? |  |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Camellia sinensis | 4-nitrophenol + beta-D-glucose | - |
? | |
| additional information | no activity with 4-nitrophenyl beta-D-xylopyranoside | Camellia sinensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 41000, SDS-PAGE | Camellia sinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Camellia sinensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable below | Camellia sinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Camellia sinensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Camellia sinensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | fairly stable | Camellia sinensis |
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Release 2023.1 (January 2023)
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